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1210 electron microscope

Manufactured by JEOL
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The JEOL 1210 electron microscope is designed for high-resolution imaging and analysis of a wide range of samples. It features a LaB6 electron source and offers a resolution of up to 0.2 nanometers. The microscope is capable of both transmission electron microscopy (TEM) and scanning electron microscopy (SEM) modes.

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Lab products found in correlation

Nunc lab tek chamber slide, by Thermo Fisher Scientific (1 mentions)

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JEM-1210 transmission electron microscope (15 citations) JEOL 1210 transmission electron microscope (9 citations) JEM-1210 electron microscope (7 citations)

2 protocols using 1210 electron microscope

1

Electron Microscopy Sample Preparation

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Cell monolayers were fixed with 2.5% glutaraldehyde buffered with 0.05 M cacodylate followed by 1% osmium in 0.05 M cacodylate for 20 minutes, and then subsequently dehydrated in ethanol and embedded in Araldite (Cedar Grove, NJ) (Murray et al. 1981 (link)). Ultra thin sections were cut, mounted on grids, and imaged with a JEOL 1210 electron microscope.
Vanderpuye O.A., Bell C.L, & Murray S.A. (2016). REDISTRIBUTION OF CONNEXIN 43 DURING CELL DIVISION. Cell biology international, 40(4), 387-396.
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2

Adipocyte Differentiation and Ultrastructural Analysis

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ADSCs were differentiated to beige adipocytes on four-well Nunc Lab-Tek chamber slides (Thermo Fisher, 177437) for 21 days. Samples were fixed in 2.5% glutaraldehyde (v/v) and 5 mM CaCl2 in 0.1 cacodylate buffer and washed in DPBS, then post-fixed for 1 hour in 1% OsO4 in cacodylate buffer with 5 mM CaCl2 and 0.8% potassium ferricyanide. Samples were dehydrated in acetone, Epon-embedded and sectioned. Sections were then stained with uranyl acetate and lead citrate and TEM analysis was performed on a JEOL 1210 electron microscope. For SEM, analysis was performed on a FEI Teneo instrument.
Singh A.M., Zhang L., Avery J., Yin A., Du Y., Wang H., Li Z., Fu H., Yin H, & Dalton S. (2020). Human beige adipocytes for drug discovery and cell therapy in metabolic diseases. Nature Communications, 11, 2758.
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