The endometrial cancer cell lines HEC1A (type I, PIK3CA G1049R mutated), HEC1B (type I, PIK3CA G1049R mutated), and AN3CA (type II, PTEN loss) were obtained from the American Type Culture Collection (Manassas, VA, USA). HEC1A and HEC1A-TR cells were maintained in Dulbecco’s Modified Eagle Medium (catalog no. LM001-05; Welgene Inc., Deagu, Korea), whereas AN3CA, HEC1B, and HEC1B-TR were maintained in Minimum Essential Medium (catalog no. LM007-07; Welgene Inc., Deagu, Korea). Briefly, 10% heat-inactivated FBS (catalog no. S001-01; Welgene Inc., Deagu, Korea) and 1% 100 × P/S solution (catalog no. LS202-02; Welgene Inc.) was added to all basal media.
An3ca
Manufactured by Welgene
The AN3CA is a laboratory instrument designed for the analysis and detection of specific biomolecules. It utilizes advanced technology to provide accurate and reliable results. The core function of the AN3CA is to facilitate the identification and quantification of target analytes in research and diagnostic applications.
Automatically generated - may contain errors
Lab products found in correlation
An3ca, by American Type Culture Collection (2 mentions)
Dulbecco s modified eagle medium, by Welgene (1 mentions)
Hec1a, by Welgene (1 mentions)
Hec1b, by Welgene (1 mentions)
Heat inactivated fbs, by Welgene (1 mentions)
Hec 1a, by American Type Culture Collection (1 mentions)
Hec 1b, by American Type Culture Collection (1 mentions)
Minimum essential medium (mem), by Welgene (1 mentions)
Hep3b, by Welgene (1 mentions)
Mda mb 453, by Welgene (1 mentions)
Hepg2, by Welgene (1 mentions)
Sk hep1, by Welgene (1 mentions)
Antibiotic antimycotic solution, by Welgene (1 mentions)
Rpmi 1640, by Welgene (1 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
Dulbecco modified eagle medium (dmem), by Welgene (1 mentions)
2 protocols using an3ca
1
Endometrial Cancer Cell Line Cultivation
2
Cell Culture Protocol for Cancer Research
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AN3-CA and J82 cells were obtained from ATCC (Manassas, VA, USA). KMS-11 cells were purchased from JCRB. HEP3B, HUH7, HEPG2, MDA-MB-453 and SK-HEP1 cells were purchased from KCLB (Seoul, Korea). HEP3B, AN3-CA, MDA-MB-453 and SK-HEP1 cells were cultured in DMEM (#LM001-05, Welgene). HUH7, HEPG2, J82 and KMS-11 cells were cultured in RPMI1640 (#LM011-01, Welgene). The culture media were supplemented with 10% fetal bovine serum (#S001-01, Welgene), antibiotic-antimycotic solution (#LS203-01, Welgene) containing 10,000U/mL penicillin. The cells were maintained in a humidified atmosphere containing 5% CO2 at 37 °C.
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