Alexa fluor 488 anti mouse cd11b

Manufactured by BioLegend

Alexa Fluor 488 anti-mouse CD11b is a fluorescently labeled antibody that binds to the mouse CD11b protein. CD11b is a cell surface marker expressed on various immune cells, including monocytes, macrophages, and granulocytes. The Alexa Fluor 488 dye attached to the antibody allows for the detection and analysis of CD11b-positive cells using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Horseradish peroxidase hrp conjugated anti rabbit secondary antibody, by Cell Signaling Technology (2 mentions) Freund s incomplete adjuvant ifa, by MP Biomedicals (2 mentions) Complete adjuvant cfa, by MP Biomedicals (2 mentions) Antibodies specific for phospho p38 thr180 tyr182 and p38, by Cell Signaling Technology (2 mentions) Nickel 2 chloride, by Fujifilm (2 mentions) Pe anti mouse 1 a 1 e, by BioLegend (2 mentions) Pe anti mouse f4 80, by BioLegend (2 mentions) Pe cyanine7 anti mouse cd4, by BioLegend (1 mentions) Fitc anti mouse cd3, by BioLegend (1 mentions) Purified rat anti mouse cd16 cd32 mouse fc block, by BD (1 mentions) Pe anti mouse cd8a, by BioLegend (1 mentions) Apc cy7 rat anti mouse cd45, by BD (1 mentions) Readidrop propidium iodide, by Bio-Rad (1 mentions) 7 aad viability staining solution, by Thermo Fisher Scientific (1 mentions) Fitc anti mouse cd11c, by BioLegend (1 mentions) Alexa fluor 488 secondary antibody, by Abcam (1 mentions) Sodium pyruvate, by Thermo Fisher Scientific (1 mentions) Apc anti mouse f4 80, by BioLegend (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions)

3 protocols using alexa fluor 488 anti mouse cd11b

Sema3A Signaling in Immune Responses

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Nickel(II) Chloride was obtained from Wako Pure Chemical Corporation. Freund’s incomplete adjuvant (IFA) and complete adjuvant (CFA) were from MP Biomedicals. The primary antibody specific for Sema3A was from Abcam. Antibodies specific for phospho-p38 (Thr180/Tyr182) and p38 were obtained from Cell Signaling Technology. Rabbit antibody to GAPDH was obtained from Osenses. Rabbit antibody to β-actin was obtained from Bioss. Horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibody were obtained from Cell Signaling. FITC anti-mouse CD11c, Alexa Fluor 488 anti-mouse CD11b, PE anti-mouse F4/80, PE anti-mouse I-A/I-E, FITC anti-mouse CD3, PE/Cyanine7 anti-mouse CD4 and PE anti-mouse CD8a were purchased from BioLegend. APC-Cy™7 Rat Anti-Mouse CD45 and Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) were from BD Biosciences. 7-AAD Viability Staining Solution was from Invitrogen. Anti-rabbit Alexa Fluor 488 secondary antibody was from Abcam. Readidrop^TM Propidium Iodide was from Bio-Rad Laboratories. The recombinant mouse Sema3A were purchased from R&D Systems.

Liu L., Watanabe M., Minami N., Yunizar M.F, & Ichikawa T. (2022). Semaphorin 3A: A potential target for prevention and treatment of nickel allergy. Communications Biology, 5, 671.

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Quantification of Inflammasome Activation in Macrophages

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RPMI 1640, penicillin/streptomycin solution, and sodium pyruvate were purchased
from Invitrogen, Life Technologies. FBS was purchased from Gibco, Life Technologies. ATP,
murine IL-1β Ab, murine β-actin Ab were purchased from Sigma Aldrich.
Antibodies for p-BAD, p-STAT3 and STAT3 were purchased from Cell Signalling. Alexa Fluor
488 anti-mouse CD11b and APC anti-mouse F4/80 were purchased from Biolegend. P2X7 Ab was
from Alomone. Fluorochrome-conjugated secondary Ab linked to Alexa Fluor 488 was from Life
Technologies. Caspase-1 Ab was purchased from Santa Cruz Biotechnology. BEL was from
Cayman Chemical.

Morandini A.C., Ramos-Junior E.S., Potempa J., Nguyen K.A., Oliveira A.C., Bellio M., Ojcius D.M., Scharfstein J, & Coutinho-Silva R. (2014). Porphyromonas gingivalis Fimbriae Dampen P2X7-Dependent Interleukin-1β Secretion. Journal of Innate Immunity, 6(6), 831-845.

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Sema3A Regulation and Immune Response

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Nickel(II) Chloride was obtained from Wako Pure Chemical Corporation. Freund's incomplete adjuvant (IFA) and complete adjuvant (CFA) were from MP Biomedicals. The primary antibodies specific for Sema3A were from Bioss Antibodies (for Western blotting) and Abcam (for immunohistochemistry/immunocytochemistry), respectively. Antibodies specific for phospho-p38 (Thr180/Tyr182) and p38 were obtained from Cell Signaling Technology. Antibody specific for mouse TSLP was from Abcam. Rabbit antibody to GAPDH was obtained from Osenses. Alexa Fluor 488 anti-mouse CD11c, Alexa Fluor 488 anti-mouse CD11b, PE anti-mouse CD115, PE anti-mouse F4/80, PE anti-mouse I-A/I-E and Alexa Fluor 647 anti-mouse CD206 (MMR) were purchased from BioLegend. Horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibody were obtained from Cell Signaling. Anti-rabbit Alexa Fluor 488 secondary antibody was from Abcam.

Liu L., Watanabe M., Minami N., Yunizar M.F., & Ichikawa T. (2021). Semaphorin3A Promotes the Development of Nickel Allergy.

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