Single-cell mouse splenocyte suspensions were prepared 2 weeks after the final immunization. For CD49dhighCD11ahigh CD4+ T cell detection, splenocytes were incubated with anti-mouse CD4-APC/Cy7 (Biolegend, USA), anti-mouse CD49d-FITC (Biolegend, USA), and anti-mouse CD11a-PE (Biolegend, USA). To detect CD8αlowCD11ahigh T cells, cells were incubated with anti-mouse CD8α-PerCP5.5 (Biolegend, USA) and anti-mouse CD11a-PE (Biolegend, USA). Fluorescence-activated cell sorting (FACS) was performed on a FACSCalibur flow cytometer (BD Biosciences) and data were analyzed using CellQuest Pro (BD Biosciences) software.
Zhou D., Zheng H., Liu Q., Lu X., Deng X., Jiang L., Hou B., Fu Y., Zhu F., Ding Y., Xu W, & Dai J. (2019). Attenuated plasmodium sporozoite expressing MAGE-A3 induces antigen-specific CD8+ T cell response against lung cancer in mice. Cancer Biology & Medicine, 16(2), 288-298.
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