Andanti ki 67

Manufactured by Novus Biologicals

Sourced in United States

Anti-Ki-67 is a laboratory reagent used for the detection of the Ki-67 protein, a well-known marker of cellular proliferation. Ki-67 is expressed during all active phases of the cell cycle (G1, S, G2, and mitosis) but is absent in resting cells (G0). Anti-Ki-67 can be used in immunohistochemical and immunocytochemical applications to identify and quantify proliferating cells within a tissue or cell sample.

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Lab products found in correlation

Docetaxel, by Selleck Chemicals (1 mentions) Live dead viability cytotoxicity kit, by Thermo Fisher Scientific (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Quant it picogreen dsdna assay kit, by Thermo Fisher Scientific (1 mentions) Accumax, by Thermo Fisher Scientific (1 mentions) T medium, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) L cysteine, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions) Phosphate buffered saline (pbs), by Lonza (1 mentions) Anti claudin 1, by Novus Biologicals (1 mentions) Anti zo 1, by Novus Biologicals (1 mentions) Anti villin, by Santa Cruz Biotechnology (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Nis element, by Nikon (1 mentions) Cy3 conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions)

2 protocols using andanti ki 67

Docetaxel-loaded HA-SH/PEG-DA Hydrogel Assay

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Phenol red-free Dulbecco’s Modified
Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), T-medium, penicillin/streptomycin,
trypsin-EDTA, l-glutamine, LIVE/DEAD viability/cytotoxicity
kit, and Quant-iT PicoGreen dsDNA Assay kit were obtained from Life
Technologies (Grand Island, NY). Fetal bovine serum (FBS) was obtained
from Atlanta Biologicals (Flowery Branch, GA). Phosphate buffered
saline (PBS) was obtained from Lonza (Walkersville, MD). ACCUMAX was
obtained from eBioscience (San Diego, CA). Thiol-modified HA (HA-SH,
Glycosil, average M_w = 240 kDa, degree
of thiolation = 1 μmol/mg HA-SH) and poly(ethylene glycol)-diacrylate
(PEG-DA, Extralink, average M_w = 3350
Da) were obtained from BioTime Inc. (Alameda, CA). This HA-SH/PEG-DA
hydrogel system previously has been characterized extensively.^{15 (link)−17 (link)} Docetaxel was obtained from Selleck Chemicals (Houston, TX). DMSO, l-cysteine, and papain papaya latex were obtained from Sigma-Aldrich
(St. Louis, MO). Primary antibodies used were human-specific anti-nuclei
antibody from Millipore (MAB1281, Billerica, MA), anti-androgen receptor
from Santa Cruz Biotechnology (sc-816, Dallas, TX), anti-cleaved caspase-3
from Cell Signaling Technology (NB110–89717, Danver, MA), and
anti-Ki-67 from Novus Biologicals (#9664S, Littleton,
CO). Sylgard 184 poly(dimethylsiloxane) (PDMS) elastomer kit was from
Dow Corning (Midland, MI).

Fong E.L., Martinez M., Yang J., Mikos A.G., Navone N.M., Harrington D.A, & Farach-Carson M.C. (2014). Hydrogel-Based 3D Model of Patient-Derived Prostate Xenograft Tumors Suitable for Drug Screening. Molecular Pharmaceutics, 11(7), 2040-2050.

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Immunofluorescence Staining of Epithelial Markers

Cited 1 time

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Immunofluorescence analysis was performed as described previously (Chen et al., 2020b (link)). Fluorescence images were obtained with a microscope (NIS-Elements, Nikon, Tokyo, Japan). The following antibodies and reagents were used: anti-ZO-1, anti-claudin-1, and anti-Ki67 (#NB500-170, Novus Biologicals, Littleton, CO, USA), anti-Villin (#SC-58897, Santa Cruz Biotechnology); anti-p-JAK2, anti-p-STAT3, and FITC-conjugated secondary antibody (#115-545-003, Jackson Laboratory, Jackson, MS, USA); Cy3-conjugated secondary antibody (#111-165-045, Jackson Laboratory); and 4′,6-diamidino-2-phenylindole (DAPI, Sigma–Aldrich).

Chen M.J., Zhou J.Y., Chen Y.J., Wang X.Q., Yan H.C, & Gao C.Q. (2021). The in ovo injection of methionine improves intestinal cell proliferation and differentiation in chick embryos by activating the JAK2/STAT3 signaling pathway. Animal Nutrition, 7(4), 1031-1038.

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