Whole-mount immunostaining of the embryos was performed as previously described [23 (link)]. Primary antibodies used were anti-c-Kit (2B8; BD Biosciences) and biotinylated anti-CD31 (MEC13.3; BD Biosciences). The secondary antibody and labeled streptavidin used were goat anti–rat IgG-Alexa Fluor 647 (Invitrogen) and Cy3-streptavidin (Jackson ImmunoResearch Laboratories). Immunostained embryos were mounted in a 1:2 mix of benzyl alcohol and benzyl benzoate (BABB) to increase tissue transparency and analyzed using a confocal microscope (Zeiss LSM 510 Meta, Plan-Neofluar 20×/NA 0.5). Three-dimensional projections were generated from z-stacks using LSM Image Browser (Zeiss).
Biotinylated anti cd31 mec13.3
Manufactured by BD
Biotinylated anti-CD31 (MEC13.3) is a monoclonal antibody that recognizes the mouse CD31 (PECAM-1) cell surface antigen. CD31 is an adhesion molecule expressed on the surface of endothelial cells, platelets, and certain leukocyte subsets.
Automatically generated - may contain errors
Lab products found in correlation
Anti c kit 2b8, by BD (1 mentions)
Alexa fluor 647 goat anti rat igg, by Thermo Fisher Scientific (1 mentions)
Cy3 streptavidin, by Jackson ImmunoResearch (1 mentions)
Lsm image browser, by Zeiss (1 mentions)
Prolong gold antifade reagent, by Thermo Fisher Scientific (1 mentions)
Streptavidin biotin blocking kit, by Vector Laboratories (1 mentions)
2 protocols using biotinylated anti cd31 mec13.3
1
Whole-mount Immunostaining of Embryos
2
Immunohistological Analysis of Ear Sections
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunohistological analysis was performed essentially as described previously (Nagakubo et al., 2016) . Briefly, the ear sections were blocked with 1% donkey serum in phosphate-buffered saline containing 2% BSA for 15 minutes and by a Streptavidin/Biotin Blocking Kit (Vector Laboratories, Burlingame, CA) for 15 minutes. Sections were then incubated overnight with goat anti-CCL17 (Santa Cruz Biotechnology, Dallas, TX) or rabbit anti-CCL22 (Peprotech, Rocky Hill, NJ) at 4 C. After washing, sections were incubated either with Alexa Fluor 555elabeled donkey anti-goat IgG (Thermo Fischer Scientific) or Alexa Fluor 555elabeled donkey anti-rabbit IgG (Thermo Fischer Scientific) for 1 hour, and incubated overnight with biotinylated anti-CD31 (MEC13.3; BD Biosciences, San Jose, CA), followed by Alexa Fluor 647elabeled streptavidin (Thermo Fischer Scientific) for 1 hour. Finally, sections were mounted with ProLong Gold Antifade Reagent (Thermo Fischer Scientific) and observed under a spectrum confocal laser-scanning microscope (C1si; Nikon, Tokyo, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!