Western blot was performed on total lung homogenates prepared in lysis buffer containing protease and phosphatase inhibitors as previously described (19 (link)) using the following antibodies: rabbit polyclonal EC-SOD antibody (1:1000, Santa Cruz Biotechnology, Santa Cruz, CA), mouse monoclonal mouse endothelial nitric oxide synthase (eNOS) antibody (1: 500, BD Biosciences, San Jose CA), rabbit polyclonal VEGF antibody (1:500, Santa Cruz Biotechnology), rabbit monoclonal Anti-human VEGF receptor 2 and phospho-VEGF receptor 2 (1:1000, Cell Signaling, Danvers, MA), EC-SOD ( 1:1000, Santa Cruz Biotechnology) and B-actin mouse monoclonal antibody (Sigma). The species-appropriate secondary IgG antibody was used (1:10,000, Millipore, Billerica, MA).
Secondary igg antibody
Manufactured by Merck Group
The Secondary IgG antibody is a laboratory reagent used in various immunoassay techniques. It functions as a detection antibody, binding to the primary antibody that is specific to the target analyte. This allows for the amplification and visualization of the immunocomplex, facilitating the identification and quantification of the target molecule.
Automatically generated - may contain errors
Lab products found in correlation
Vegf antibody, by Santa Cruz Biotechnology (2 mentions)
Ec sod, by Santa Cruz Biotechnology (2 mentions)
Ec sod antibody, by Santa Cruz Biotechnology (2 mentions)
Anti human vegf receptor 2 and phospho vegf receptor 2, by Cell Signaling Technology (2 mentions)
β actin mouse monoclonal antibody, by Santa Cruz Biotechnology (2 mentions)
Enos antibody, by BD (2 mentions)
Tcp1 alpha primary antibody, by Abcam (2 mentions)
4 protocols using secondary igg antibody
1
Quantitative Protein Analysis of Lung Tissue
2
Quantitative Protein Analysis of Lung Tissue
3
Immunohistochemical Analysis of TCP1 Alpha
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Serial 3-μm sections from all samples were deparaffinized and rehydrated through xylenes and serial graded ethanol to water followed by antigen retrieval. These samples then were incubated overnight at 4 °C with TCP1 alpha primary antibody (Abcam Corporation; 1:200). The washed tissue samples were incubated with secondary antibody IgG (Merck Millipore; 1:300) for 30 min at room temperature (RT). Tissue slices were stained with 3,3’-diaminobenzidine and hematoxylin, and observed under an optical microscope. Finally, all images were analyzed integrated optical density (IOD) to calculate the average IOD /TCP1 positive staining area (μm 2) using Image-pro plus software.
4
Quantitative Analysis of TCP1 Alpha
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serial 3-μm sections from all samples were depara nized and rehydrated through xylenes and serial graded ethanol to water followed by antigen retrieval. These samples then were incubated overnight at 4 °C with TCP1 alpha primary antibody (Abcam Corporation; 1:200). The washed tissue samples were incubated with secondary antibody IgG (Merck Millipore; 1:300) for 30 min at room temperature (RT).
Tissue slices were stained with 3,3'-diaminobenzidine and hematoxylin, and observed under an optical microscope. Finally, all images were analyzed integrated optical density (IOD) to calculate the average IOD /TCP1 positive staining area (µm 2 ) using Image-pro plus software.
Tissue slices were stained with 3,3'-diaminobenzidine and hematoxylin, and observed under an optical microscope. Finally, all images were analyzed integrated optical density (IOD) to calculate the average IOD /TCP1 positive staining area (µm 2 ) using Image-pro plus software.
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