Mouse leptin duoset elisa

Manufactured by R&D Systems

Sourced in United States

The Mouse Leptin DuoSet ELISA is a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of mouse leptin in cell culture supernates, serum, and plasma. Leptin is a hormone primarily produced by adipose tissue that regulates energy balance and metabolism.

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Lab products found in correlation

Mouse glucagon elisa kit, by Crystal Chem (1 mentions) Mouse rat fgf 21 quantikine elisa kit, by R&D Systems (1 mentions) Ultra sensitive mouse insulin elisa kit, by Crystal Chem (1 mentions) Legendplex mouse inflammation kit, by BioLegend (1 mentions) Mouse adiponectin acrp30 duoset elisa, by R&D Systems (1 mentions)

Close spelling variants of this product

Mouse Leptin DuoSet ELISA Kit Mouse Leptin R DuoSet ELISA Mouse leptin ELISA DuoSet ELISA DuoSet Mouse Leptin DuoSets ELISAs

2 protocols using mouse leptin duoset elisa

Measuring Serum Hormone Levels in Mice

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Total ghrelin was measured in serum samples collected from live mice using the Rat/Mouse Ghrelin (total) ELISA Kit from Millipore Sigma® (Burlington, MA, USA) per manufacturer's instructions. For all other hormone measurements, serum collected during blood/tissue harvesting was used. Serum FGF21 and leptin levels were measured using the Mouse/Rat FGF‐21 Quantikine ELISA Kit and the Mouse Leptin DuoSet ELISA, respectively, from R&D Systems® (Minneapolis, MN, USA) per manufacturer's instructions. Serum glucagon and serum insulin were analysed using the Mouse Glucagon ELISA Kit and the Ultra‐Sensitive Mouse Insulin ELISA Kit, respectively, from Crystal Chem® (Elk Grove Village, IL, USA) according to the manufacturer's instructions.

Nguyen A.T., Amigo Z., McDuffie K., MacQueen V.C., Bell L.D., Truong L.K., Batchi G, & McMillin S.M. (2024). Effects of Empagliflozin‐Induced Glycosuria on Weight Gain, Food Intake and Metabolic Indicators in Mice Fed a High‐Fat Diet. Endocrinology, Diabetes & Metabolism, 7(2), e00475.

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Plasma Biomarker Quantification in Mice

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Adiponectin (Mouse Adiponectin/Acrp30 DuoSet ELISA, DY1119, R&D Systems), leptin (Mouse Leptin DuoSet ELISA, DY498-05, R&D Systems) and insulin (U-PLEX Mouse Insulin Assay, MSD) concentrations were determined by immunoassay. Plasma cytokines were quantified with LEGENDplex Mouse Inflammation kit (Biolegend) according to the manufacturer’s instructions.

Orliaguet L., Ejlalmanesh T., Humbert A., Ballaire R., Diedisheim M., Julla J.B., Chokr D., Cuenco J., Michieletto J., Charbit J., Lindén D., Boucher J., Potier C., Hamimi A., Lemoine S., Blugeon C., Legoix P., Lameiras S., Baudrin L.G., Baulande S., Soprani A., Castelli F.A., Fenaille F., Riveline J.P., Dalmas E., Rieusset J., Gautier J.F., Venteclef N, & Alzaid F. (2022). Early macrophage response to obesity encompasses Interferon Regulatory Factor 5 regulated mitochondrial architecture remodelling. Nature Communications, 13, 5089.

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