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Ezfast kit

Manufactured by Phenomenex
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The EZfast kit is a sample preparation tool designed for fast and efficient extraction of target analytes from complex matrices. It provides a streamlined workflow to simplify the sample preparation process.

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Lab products found in correlation

Elisas, by Crystal Chem (2 mentions)

2 protocols using ezfast kit

1

Fasting Insulin and Amino Acid Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Insulin levels (6-hour fasting) were measured with ELISAs (Crystal Chem) following the manufacturer’s instructions. At week 17 post VSG/Sham surgery in mice (Figures 3 and 7) and 2 weeks post VSG/Sham surgery in rats (Figure 1), food was removed at the start of dark phase (18:00hr, 12:12 light-dark cycle), and mice were fasted overnight. Plasma amino acid level analysis was performed at Michigan Regional Comprehensive Metabolomics Resource Core (University of Michigan, Ann Arbor). Amino acid levels were analyzed in overnight fasted plasma using the Phenomenex EZfast kit. Samples are extracted, semi- purified, derivatized and measured by EI-GCMS using norvaline as an internal standard for normalization. All blood was collected via tail vein in EDTA-coated tubes.
Kramer N.B., Evers S.S., Shin J.H., Silverwood S., Wang Y., Burant C.F., Sandoval D.A, & Seeley R.J. (2020). The Role of Elevated Branched-Chain Amino Acids in the Effects of Vertical Sleeve Gastrectomy to Reduce Weight and Improve Glucose Regulation. Cell reports, 33(2), 108239.
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2

Fasting Insulin and Amino Acid Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Insulin levels (6-hour fasting) were measured with ELISAs (Crystal Chem) following the manufacturer’s instructions. At week 17 post VSG/Sham surgery in mice (Figures 3 and 7) and 2 weeks post VSG/Sham surgery in rats (Figure 1), food was removed at the start of dark phase (18:00hr, 12:12 light-dark cycle), and mice were fasted overnight. Plasma amino acid level analysis was performed at Michigan Regional Comprehensive Metabolomics Resource Core (University of Michigan, Ann Arbor). Amino acid levels were analyzed in overnight fasted plasma using the Phenomenex EZfast kit. Samples are extracted, semi- purified, derivatized and measured by EI-GCMS using norvaline as an internal standard for normalization. All blood was collected via tail vein in EDTA-coated tubes.
Kramer N.B., Evers S.S., Shin J.H., Silverwood S., Wang Y., Burant C.F., Sandoval D.A, & Seeley R.J. (2020). The Role of Elevated Branched-Chain Amino Acids in the Effects of Vertical Sleeve Gastrectomy to Reduce Weight and Improve Glucose Regulation. Cell reports, 33(2), 108239.
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