Log In Sign up
The largest database of trusted experimental protocols

4 hydroxy 2 nonenal

Manufactured by Cayman Chemical
Visit Supplier
Sourced in United States

4-hydroxy-2-nonenal is a laboratory reagent. It is a naturally occurring lipid peroxidation product.

Automatically generated - may contain errors

Lab products found in correlation

4 hydroxy 2 hexenal, by Cayman Chemical (2 mentions) Octanal, by Merck Group (1 mentions) Nonanal, by Merck Group (1 mentions) Hexanal, by Merck Group (1 mentions) 2 hexenal, by Merck Group (1 mentions) 2 4 nonadienal, by Tokyo Chemical Industry (1 mentions) 4 5 epoxy 2 decenal, by Cayman Chemical (1 mentions) Propanal, by Merck Group (1 mentions) Pentanal, by Merck Group (1 mentions) Heptanal, by Merck Group (1 mentions) Decanal, by Merck Group (1 mentions) Dodecanal, by Merck Group (1 mentions) Crotonaldehyde, by Tokyo Chemical Industry (1 mentions) Glyoxal, by Tokyo Chemical Industry (1 mentions) Heptadecanal, by Tokyo Chemical Industry (1 mentions) Hexadecanal, by Tokyo Chemical Industry (1 mentions) Octadecanal, by Tokyo Chemical Industry (1 mentions) Pentadecanal, by Tokyo Chemical Industry (1 mentions) Tetradecanal, by Tokyo Chemical Industry (1 mentions) Undecanal, by Merck Group (1 mentions)

Spelling variants of this product

4-hydroxy-2-nonenal (HNE) (3 citations) 4-Hydroxy-trans-2-nonenal (4-HNE) (2 citations)

4 protocols using 4 hydroxy 2 nonenal

1

Analytical Reagents for Aldehyde Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
All chemicals purchased were of analytical grade (ACS grade) and were used as received without any additional purification. Crotonaldehyde, dansyl hydrazine (DH), 2,4-decadienal, glyoxal, heptadecanal, hexadecanal, 2,4-nonadienal, octadecanal, pentadecanal, and tetradecanal were purchased from Tokyo Chemical Industry (Tokyo, Japan). p-Toluenesulfonic acid (p-TsOH), butanal, decanal, dodecanal, 2-hexenal, hexanal, 2-heptenal, heptanal, octanal, 2-nonenal, nonanal, pentanal, propanal, tridecanal, and undecanal were obtained from Sigma-Aldrich (St. Louis, MO, USA). 4,5-Epoxy-2-decenal, 4-hydroxy-2-hexenal, 4-hydroxy-2-nonenal, and 4-oxo-2-nonenal were purchased from the Cayman Chemical Company (Ann Arbor, MI, USA). All other chemicals were obtained from Wako Pure Chemical Industries (Osaka, Japan). 8-Heptadecenal, 8,11-heptadecadienal, and 8,11,14-heptadecatrienal were synthesized following the published method [31 (link)].
Mure K., Tomono S., Mure M., Horinaka M., Mutoh M., Sakai T., Ishikawa H, & Wakabayashi K. (2021). The Combination of Cigarette Smoking and Alcohol Consumption Synergistically Increases Reactive Carbonyl Species in Human Male Plasma. International Journal of Molecular Sciences, 22(16), 9043.
+ Open protocol
+ Expand
2

Heterologous Expression of AKR1B15 in E. coli

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols

E. coli BL21(DE3) strain was obtained from Novagen, while plasmids pBB540 and pBB542 (containing the chaperone-coding genes grpE, clpB and dnaK, dnaJ, groESL, respectively), were a kind gift from Dr. A. de Marco [22 (link)]. The pET-28a vector containing the cDNA coding for isoform 2 of AKR1B15 (UniProt ID: C9JRZ8-2) had been described by Salabei et al. [6 (link)]. Tolrestat and sorbinil were generously provided by Prof. T.G. Flynn and Pfizer, respectively, whereas JF0064 (2,2’,3,3’,5,5’,6,6’-octafluoro-4,4’-biphenyldiol) was obtained from Sigma-Aldrich. Trans-2-hexenal and 4-hydroxy-2-nonenal were commercially obtained from Cayman Chemical. All other reagents, including substrates, were purchased from Sigma-Aldrich unless otherwise indicated.
Giménez-Dejoz J., Kolář M.H., Ruiz F.X., Crespo I., Cousido-Siah A., Podjarny A., Barski O.A., Fanfrlík J., Parés X., Farrés J, & Porté S. (2015). Substrate Specificity, Inhibitor Selectivity and Structure-Function Relationships of Aldo-Keto Reductase 1B15: A Novel Human Retinaldehyde Reductase. PLoS ONE, 10(7), e0134506.
+ Open protocol
+ Expand
3

Enzymatic Digestion and Oxidative Stress Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pepsin (2188 U mg -1 ) from porcine gastric mucosa, α-amylase (1333 U mg -1 ) from Bacillus sp., trypsin from bovine pancreas, Patent Blue V sodium salt, ethylenediaminetetraacetic acid (EDTA), disodium salt dihydrate, ascorbic acid, 1,1,3,3-tetraethoxypropane and 2,4-dinitrophenylhydrazine (DNPH) were purchased from Sigma-Aldrich (Stockholm, Sweden). Brij 35 was obtained from Fisher Scientific (Acros Organics, Västra Frölunda, Sweden). Lipase F-AP 15 (150 U mg -1 ) from Rhizopus oryzae was purchased from Amano Enzyme Inc. (Nagoya, Japan). Fresh porcine bile was obtained from TNO (Zeist, The Netherlands). Pancreatin (Pancrex-Vet, Pfizer, Karlsruhe, Germany) was purified by centrifugation (9000g, 20 min, 4 °C) before use. Wheat flour bread containing 1% vegetable oil (0.4% saturated) was bought in a local supermarket. 4-Hydroxy-2-hexenal (HHE) and 4-hydroxy-2-nonenal (HNE) were purchased from Cayman Chemicals (Michigan, USA). All other chemicals used were of analytical grade.
Larsson K., Tullberg C., Alminger M., Havenaar R, & Undeland I. (2016). Malondialdehyde and 4-hydroxy-2-hexenal are formed during dynamic gastrointestinal in vitro digestion of cod liver oils. Food & function, 7(8).
+ Open protocol
+ Expand
4

Computational Toxicity Evaluation of Empagliflozin

Check if the same lab product or an alternative is used in the 5 most similar protocols
All chemicals were purchased from Sigma Aldrich Co, except 2-hydroxy-tetrahydrofuran from Santa Cruz Biotechnology Inc (Dallas, TX), 4-hydroxy-2-nonenal from Cayman Chemical Co (Ann Arbor, MI), 2-phenyl propenal from Novel Chemical Solutions (Crete, NE), CTA from Crescent Chemicals Co (Islandia, NY), and empagliflozin and M466/2 from Boehringer Ingelheim Pharmaceuticals Incorporated (Ridgefield, CT). M466/2 was synthesized and characterized by nuclear magnetic resonance to confirm the correct structure. 4 Computational Structure-Toxicity Relationship Analysis Empagliflozin, M466/2, and other putative aldehyde intermediates were submitted to computational structure-activity relationship (SAR) analysis to initially evaluate potential structural alerts for toxicity, with the main focus on potential genotoxicity. Analyses were conducted using the following independent computer models: DEREK (Lhasa, Leeds, UK, www.Lhasalimited.org) and CASE Ultra (MultiCASE, Beechwood, OH, www.Multicase.com). In addition, public domain databases were searched for structurally similar compounds (Tanimoto method) with toxicity data (Leadscope; www.Leadscope.com).
Smith J.D., Huang Z., Escobar P.A., Foppiano P., Maw H., Loging W., Yu H., Phillips J.A., Taub M, & Ku W.W. (2017). A Predominant Oxidative Renal Metabolite of Empagliflozin in Male Mice Is Cytotoxic in Mouse Renal Tubular Cells but not Genotoxic. International journal of toxicology, 36(6).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!