Anti il 1

For routine work, phosphate-buffered saline (PBS), RPMI 1640, formalin, and histological alcohol were obtained through ThermoFisher (Pittsburgh, PA). For humane animal sacrifice, Euthasol was obtained from Butler-Schein (Dublin, OH). Fetal bovine serum (FBS) used was from Atlanta Biologicals (Flowery Branch, GA). Pluronic F127 was from Sigma (St.Louis, MO). Liberase TL and DNaseI were from Roche Diagnostics (Indianapolis, IN). Percoll was from Santa Cruz Biotech (Santa Cruz, CA). All reagents (antibodies and bead-conjugated avidin) for magnet assisted cell sorting (MACS) were purchased from Miltenyi Biotech (Auburn, CA). The following antibodies were obtained from eBioscience (San Diego, CA): anti-CD16/32, anti-CD326-APC. The following antibodies were obtained from BioLegend (San Diego, CA): anti-CD45-Alexa Fluor 700, anti-Ki67, anti-IL-1ß, anti-rabbit IgG-DyLight 488, anti-hamster IgG-DyLight 594, and anti-rat IgG-DyLight 594. Anti-proSPC was obtained from Millipore (Billerica, MA). TRI reagent was from Molecular Research Center (Cincinnati, OH). RNeasy columns were from Qiagen (Valencia, CA). qRT-PCR was carried out using Brilliant III SYBR Green Master Mix (Agilent, Santa Clara, CA) and primers were synthesized by Integrated DNA Technologies (Coralville, IA).