Gantrez an 139

Polyethylene glycol (PEG, MW 10,000 Da), ibuprofen sodium, poly(vinyl alcohol) (PVA, MW 31,000–50,000 g/mol), polyvinylpyrrolidone (PVP, MW 40,000 g/mol), alginic acid sodium salt and the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) cell viability reagent were purchased from Sigma Aldrich, Dorset, UK. Eudragit® S (MW 125,000 g/mol) and Eudragit® L (MW 125,000 g/mol) were obtained from Rohm GmbH & Co.KG, Pharma Polymers, Darmstadt, Germany. Poly(lactic acid) (PLA) was purchased from Futerro, Escanaffles, Belgium. Isocratic HPLC grade methanol and acetonitrile were purchased from VWR International, East Grinstead, UK. L-132 lung epithelial cells were purchased from the American Type Culture Collection (ATCC) and EpiSkin™ was purchased from Skin Ethic Laboratories, Lyon, France. The human IL-1α ELISA kit and Bradford assay kit were purchased from Pierce, Rockford, IL, USA. Gantrez® AN-139, a co-polymer of methyl vinyl ether and maleic anhydride (PMVE/MAH, MW 1,080,000 Da) and Gantrez® MS-955, a mixed sodium and calcium salt of methyl vinyl ether and maleic anhydride copolymer (PVM/MA, MW 1,000,000 Da) were gifts from Ashland, Kidderminster, UK. All other chemicals used were of analytical reagent grade.

Gantrez^® AN‐139 (methylvinylether and maleic anhydride copolymer) (M_w = 1.0 × 10⁶ Da) and Gantrez^® S‐97 (acid form of methylvinylether and maleic anhydride copolymer) (M_w = 1.2 × 10⁶ Da), were provided by Ashland (Tadworth, Surrey, UK). Poly(ethyleneglycol) (PEG) 10,000 Daltons was obtained from Sigma‐Aldrich (Poole, Dorset, UK). Parafilm^® M, an olefin‐type flexible thermoplastic sheet (127 μm thickness), was obtained from Brand GMBH (Wertheim, Germany).

Gantrez^® AN-139, a copolymer of methylvinylether and maleic anhydride (PVME/MA) was a gift from Ashland, Kidderminster, UK. Methylene blue was purchased from Sigma–Aldrich, Steinheim, Germany. Sodium hydroxide was obtained from Fisher Scientific UK Limited, Loughborough, Leicestershire, UK. Parafilm M™ was purchased from BRAND GMBH, Wertheim, Germany. All other chemicals used were of analytical reagent grade.