Recombinant human IL2 was obtained through the AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH from Dr. Maurice Gately, Hoffmann - La Roche Inc. Concanavalin A (ConA) was purchased from Sigma-Aldrich (St. Louis, MO). Streptavidin-PerCP was purchased from BD Biosciences PharMingen (San Diego, CA). Mouse anti-feline CD25 (mAb 9F23) was kindly provided by K. Ohno (University of Tokyo, Tokyo, Japan). Mouse anti-feline CD4 (mAb 30A) and CD8+ (mAb 3.357) were developed in our laboratory (33 (link)). PE conjugated rat anti-mouse FoxP3 (FJK-16s) used for flow cytometry analysis was purchased from eBioscience (San Diego, CA). ChIP grade anti-FoxP3 antibody (ab2481) used for ChIP immunoprecipitation was purchased from Abcam (Cambridge, MA). Curcumin (Product #C27727) and gemcitabine (Product #G6423) were purchased from Sigma-Aldrich.
Pe conjugated rat anti mouse foxp3 fjk 16s
Manufactured by Thermo Fisher Scientific
The PE conjugated rat anti-mouse FoxP3 (FJK-16s) is a flow cytometry reagent used for the detection and analysis of mouse FoxP3-expressing cells, such as regulatory T cells (Tregs). FoxP3 is a transcription factor that is specifically expressed in Tregs and is crucial for their development and function.
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2 protocols using pe conjugated rat anti mouse foxp3 fjk 16s
1
Feline T-cell Activation and Regulatory Assay
2
Feline Regulatory T Cell Characterization
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Recombinant human IL2 was obtained through the AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH from Dr. Maurice Gately, Hoffmann - La Roche Inc. LPS and Concanavalin A (ConA) were purchased from Sigma-Aldrich (St. Louis, MO). Anti-mouse IgG coated magnetic Dynabeads® M-450 were purchased from Dynal (Great Neck, NY). Streptavidin–PerCP was purchased from BD Biosciences PharMingen (San Diego, CA). Anti-TGFβ1 (MAB240) was purchased from R&D Systems (Minneapolis, MN) and conjugated to allophycocyanin (APC) or left unconjugated for blocking studies; PE-conjugated anti-TGFβ-RII (FAB241P), neutralizing anti-TGFβRII (AF-241-NA), and recombinant human TGFβ1 (240-B) were also purchased from R&D Systems. Mouse anti-feline CD25 (mAb 9 F23) was kindly provided by K. Ohno (University of Tokyo, Tokyo, Japan). Anti-CD21 was purchased from Serotec (Raleigh, NC). Mouse anti-feline CD4 (mAb 30A) and CD8 (mAb 3.357) were developed in our laboratory [43 (link)]. PE conjugated rat anti-mouse FoxP3 (FJK-16 s) was purchased from eBioscience (San Diego, CA). FITC-conjugated anti-GARP IgG2b monoclonal antibody (LRRC32, Plato-1) was purchased from Enzo life Sciences (Ann Arbor, MI).
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