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Risc free sirna

Manufactured by Horizon Discovery
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RISC-free siRNA is a type of small interfering RNA (siRNA) that is designed to silence target genes without activating the RNA interference (RNAi) pathway. It functions by directly inhibiting mRNA translation, leading to the downregulation of the target gene expression.

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Lab products found in correlation

Sirna oligos, by Horizon Discovery (1 mentions) Dharmafect 1 reagent, by Horizon Discovery (1 mentions) In cell analyzer 2200, by GE Healthcare (1 mentions) Lipofectamine 2000, by Horizon Discovery (1 mentions) Dharmafect 1, by Horizon Discovery (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

RISC-free Co-siRNA SiGENOME RISC-Free Control siRNA SiGENOME RISC‐Free siRNA SiGLO RISC-free siRNA RISC-free control siRNA SiGLO RISC-free control siRNA SiRNAs

3 protocols using risc free sirna

1

siRNA-mediated Knockdown of Key Regulators

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siRNA oligos against human ASPP2, Lats1, Lats2, Par3, YAP and RISC-Free siRNA were purchased from Dharmacon. Cells were transfected with the indicated siRNA oligos at a final concentration of 35 nM using Dharmafect 1 reagent (Dharmacon) for 3 to 5 days, according to the manufacturer's instructions.
Royer C., Koch S., Qin X., Zak J., Buti L., Dudziec E., Zhong S., Ratnayaka I., Srinivas S, & Lu X. (2014). ASPP2 Links the Apical Lateral Polarity Complex to the Regulation of YAP Activity in Epithelial Cells. PLoS ONE, 9(10), e111384.
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2

Quantifying Golgi-to-ER Protein Localization

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To determine the change in localization of the B4GT-EGFP-HT2 construct from the Golgi to the ER, HeLa cells were plated in 384-well plates in culture medium containing doxycycline, lipofectamine2000 and a RISC-free siRNA (Dharmacon). After 24 h, culture medium was exchanged and after an additional 24 h incubation, cells were treated for 4 h with HyT36 or DMSO. Cells were fixed with 4% PFA in PBS, washed 3× with PBS, stained with Hoechst, and imaged with a GE IN Cell analyzer 2200, with five fields of view per well. Images were analyzed with Cell Profiler (Carpenter et al., 2006 (link)). Cells were identified first by the nuclear stain and following by the GFP signal. The integrated intensity and the CV of the GFP signal were determined. The mean per well was calculated and used to generate the mean and SD over 16 wells for each condition. Data were plotted in Prism (GraphPad).
Hellerschmied D., Serebrenik Y.V., Shao L., Burslem G.M, & Crews C.M. (2019). Protein folding state-dependent sorting at the Golgi apparatus. Molecular Biology of the Cell, 30(17), 2296-2308.
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3

Transfection of Huh7 Cells with siRNA

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Commercially synthesized siRNA oligonucleotides (IDT) were annealed to a final concentration of 20 μM (Pager et al., 2013 (link)). The following siRNA oligonucleotides were used: the RISC-free siRNA (Dharmacon, D-001220-01-05), siGL2 sense-strand 5′-CGUACGCGGAAUACUUCGAUU-3′ and siGL2 antisense-strand 5′-UCGAAGUAUUCCGCGUACGUU-3′, and siDDX6 sense-strand 5′-GCAGAAACCCUAUGAGAUUUU-3′ and siDDX6 antisense-strand 5′-AAUCUCAUAGGGUUUCUGCUU-3′. Huh7 were seeded in a 60 mm or 100 mm cell culture dish, and 24 h later transfected with siRNAs (25 nM or 50 nM) alone using either Dharmafect I (Dharmacon) or Lipofectamine 2000 (Invitrogen), or with 25 nM siRNAs and 1 μg plasmid DNA using Lipofectamine 2000 (Invitrogen). Transfection reactions were prepared according to the manufacturer’s specifications.
Biegel J.M., Henderson E., Cox E.M., Bonenfant G., Netzband R., Kahn S., Eager R, & Pager C.T. (2017). Cellular DEAD-box RNA helicase DDX6 modulates interaction of miR-122 with the 5′ untranslated region of hepatitis C virus RNA. Virology, 507, 231-241.
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