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Antibiotic and antimycotic solution

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1X antibiotic and antimycotic solution is a sterile, liquid media supplement used to inhibit the growth of bacteria, fungi, and other microorganisms in cell culture applications. It contains a combination of antibiotics and antifungal agents to provide broad-spectrum protection against contamination.

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Lab products found in correlation

Hek293t, by American Type Culture Collection (1 mentions) Hs578t, by American Type Culture Collection (1 mentions) Bt549, by American Type Culture Collection (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) Mda mb 468, by American Type Culture Collection (1 mentions) Dulbecco modified eagle medium (dmem), by Corning (1 mentions) L lysine, by Merck Group (1 mentions) Dmem for silac, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Antibiotic-antimycotic solution (145 citations) Antibiotic-antimycotic (68 citations) Antibiotics/Antimycotics (5 citations) Antibiotic and antimycotic (3 citations)

2 protocols using antibiotic and antimycotic solution

1

Culturing Human Breast Cancer and Kidney Cells

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Human breast cancer MDA-MB-231, MDA-MB-468, MCF7, BT-549, Hs578T and embryonic kidney HEK-293T cells were purchased from American Type Culture Collection and cultured with Dulbecco’s Modified Eagle’s Medium (DMEM, Cat# 10–013-CV, Corning) containing fetal bovine serum (10%, Cat# 10437028, ThermoFisher) and 1X antibiotic and antimycotic solution (Cat# 30–004-CI, Corning).
Wang Y.C., Kelso A.A., Karamafrooz A., Chen Y.H., Chen W.K., Cheng C.T., Qi Y., Gu L., Malkas L., Taglialatela A., Kung H.J., Moldovan G.L., Ciccia A., Stark J.M, & Ann D.K. (2023). Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination. Cell reports, 42(4), 112296.
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2

Arginine-free media preparation

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Arginine-free media for BT-549, HEK-293T, Hs578T, MCF7, MDA-MB-231 and MDA-MB-468 cells were prepared from DMEM for SILAC (Cat# 88364, ThermoFisher) supplemented with dialyzed FBS (10%, Cat# 26400–044, ThermoFisher), 1X antibiotic and antimycotic solution (Cat# 30–004-CI, Corning), and L-lysine (146.2 μg/ml, Cat# L-9037, Millipore Sigma). For arginine removal, cells were washed with warm arginine-free medium once then incubated in arginine-free medium for indicated times.
Wang Y.C., Kelso A.A., Karamafrooz A., Chen Y.H., Chen W.K., Cheng C.T., Qi Y., Gu L., Malkas L., Taglialatela A., Kung H.J., Moldovan G.L., Ciccia A., Stark J.M, & Ann D.K. (2023). Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination. Cell reports, 42(4), 112296.
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