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Cholesterol quantification kit mak043

Manufactured by Merck Group
Sourced in United States

The Cholesterol Quantification Kit (MAK043) is a biochemical assay kit designed to quantify total cholesterol levels in a sample. The kit utilizes an enzymatic reaction to colorimetrically determine the concentration of cholesterol present. It provides a simple, quick, and reliable method for cholesterol measurement.

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2 protocols using cholesterol quantification kit mak043

1

Synthesis and Characterization of Dopamine-Functionalized PVA Hydrogels

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Polyvinyl alcohol (PVA), formaldehyde, sulfuric acid, starch, n-pentane, 1,4-dioxane, trihydroxymethyl-aminomethane (Tris) and hydrochloric acid (HCl) were obtained from Aladdin Bio-technology (Shanghai, China). Dopamine hydrochloride (DA), silver nitrate (AgNO3), methylene blue, glucose assay kit (GAGO20), and cholesterol quantification kit (MAK043) were acquired from Sigma-Aldrich (St. Louis, MO, USA). Escherichia coli (E. coli) was purchased from Fu Xiang Biological Technology (Shanghai, China). The blood glucose meter (Nipro Diagnostics Inc., Coral Gables, FL, USA) and cholesterol meter (BeneCheck, Taiwan) were procured from a local drugstore (Putian, China). All other chemicals used were of analytical or pharmaceutical grade.
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2

Quantifying Serum and Liver Cholesterol

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An aliquot of nonfasted serum as described earlier was used for cholesterol (Cholesterol Quantification Kit MAK043, Sigma-Aldrich®, St Louis, MO, USA) and triglyceride quantification (Triglyceride Quantification Assay Kit ab65336, Abcam®, Cambridge, MA, USA). Liver cholesterol was also isolated from nonfasted liver tissue and quantified according to the manufacturer's protocol. Measurements were taken from 5 control and 10 MNR litters, 3-6 pups/litter.
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