Exionlc hplc system

Manufactured by AB Sciex

Sourced in Canada, Japan, United States

The ExionLC HPLC system is a high-performance liquid chromatography (HPLC) instrument designed for analytical and preparative separations. It features a modular design, allowing for the integration of various components such as pumps, autosamplers, and detectors to meet specific analytical requirements. The ExionLC HPLC system is capable of delivering precise and reliable performance in a wide range of applications.

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Lab products found in correlation

Hyperrez xp organic acid column, by Thermo Fisher Scientific (1 mentions) Qtrap 6500 mass spectrometer, by AB Sciex (1 mentions) Api 5500 qtrap mass spectrometer, by AB Sciex (1 mentions) Multiquant 3, by AB Sciex (1 mentions) C18 pre column, by Phenomenex (1 mentions) Luna c18 column, by Phenomenex (1 mentions) Analyst 1, by AB Sciex (1 mentions) Inertsil sil 100a column, by GL Sciences (1 mentions) 4000 qtrap mass spectrometer, by AB Sciex (1 mentions) Qtrap 4500, by AB Sciex (1 mentions) Liaison xl analyzer, by DiaSorin (1 mentions)

4 protocols using exionlc hplc system

Tracing Glycolate Fate in Microbes

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To follow the fate of glycolate, 1 mM ¹³C-labeled glycolic acid (1,2-13C2; 99% pure; Cambridge Isotope Lab) was added to the shake flask cultures after grown for 2 days under the conditions described previously. Subsequently, the cells were cultivated for 2 days, the extracellular concentration of ¹³C-labeled glycolate were analyzed by LC–MS.
100 μL aliquots of culture supernatants were mixed with 900 μL methanol and centrifuged for 5 min at 20,000×g. 500 μL aliquots of supernatants were lyophilized and sequentially resuspended in 500 μL of water.
The extracellular concentrations of ¹³C-labeled glycolate were analyzed using an ExionLC HPLC system (AB SCIEX) coupled with a 6500 Q-TRAP mass spectrometer (AB SCIEX). Glycolate were separated with a HyperREZ XP Organic acid column (10 cm length, 7.7 mm diameter, 8 µm particle size; Thermo ScientificTM) using H₂O as mobile phase. The column was maintained at 40 °C with a solvent flow rate of 0.4 mL min⁻¹. Injection volume was 10 µL. Electrospray ionization was used in negative mode.

Zhou J., Yang F., Zhang F., Meng H., Zhang Y, & Li Y. (2021). Impairing photorespiration increases photosynthetic conversion of CO2 to isoprene in engineered cyanobacteria. Bioresources and Bioprocessing, 8(1), 42.

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LC-MS/MS for Quantitative Analysis

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The LC-MS/MS system consisted of an AB Sciex ExionLC HPLC system connected to an AB Sciex API 5500 Qtrap mass spectrometer (AB Sciex, Concord, ON, Canada). Analyst 1.6.3 software (AB Sciex) controlled the system, and the data was processed by Multiquant 3.0.2 software (AB Sciex). Liquid chromatography was performed as described by Cerkvenik-Flajs (2 (link)) in a Luna C₁₈ column with 3 μm particle size and with 50 × 2 mm dimensions connected to a C₁₈ precolumn of 4 × 2 mm (both Phenomenex, Torrance, CA, USA). The mobile phase was composed of two reagents: A was 10% methanol in 0.2 M ammonium formate and B was methanol. The composition of the mobile phase was 100% A for 2 min, then 90% B for 1 min, and after that 100% A once more until the end of the sequence. The column was operated at 40°C. The flow rate was 0.4 mL min⁻¹. The MS/MS system was operated at the following parameters: curtain gas was set at 35 psi, collision gas at the high setting, desolvation temperature at 600°C, and ion source gases 1 and 2 at 40 psi. Electrospray in positive mode was used at a voltage of 5,500 V and the electron multiplier was used at a voltage of 2,100 V. The ions were monitored in multiple-reaction monitoring mode (Table 1).

Śniegocki T., Samorek E., Sell B, & Krajewska W. (2024). Development of a method for the determination of sedatives in bovine and porcine urine and kidneys by liquid chromatography–tandem mass spectrometry. Journal of Veterinary Research, 68(1), 137-145.

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Mass Spectrometric Analysis of Triacylglycerol Hydroperoxide Isomers

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Q1 mass and product ion mass spectra in the TG 18:1_18:1_18:1;OOH isomer mixture were obtained using a 4000 QTRAP mass spectrometer (SCIEX, Tokyo, Japan). Standard TG 18:1_18:1_18:1;OOH was diluted in methanol (0.5 µM) and directly infused into the MS at a flow rate of 10 µL/min. Positive ESI was used as the ion source. MS spectra were obtained in a range of m/z 100–1000. Analytical parameters were optimized using the Analyst software (ver. 1.6.2, SCIEX, Tokyo, Japan) (Supplementary Materials).
TG 18:1_18:1_18:2;OOH and TG 18:1_18:1_18:1;OOH isomers were analyzed in multiple-reaction-monitoring (MRM) mode. The MRM transitions described in the Supplementary Materials were used. LC-MS/MS analysis was conducted using an ExionLC HPLC system (SCIEX, Tokyo, Japan) equipped with a 4000 QTRAP mass spectrometer. An Inertsil SIL-100A column (5 µm, 2.1 × 250 mm, GL Sciences Inc., Tokyo, Japan) was eluted with hexane/2-propanol/acetic acid (100:0.6:0.5, v/v/v) at 0.2 mL/min (40 °C). A post-column solvent consisting of methanol/2-propanol (1:1, v/v) containing 0.2 mM sodium acetate was mixed with the eluent at 0.2 mL/min to promote ionization [29 (link)]. TG 18:1_18:1_18:2;OOH and TG 18:1_18:1_18:1;OOH isomers were quantitated with external standard curves.

Takahashi H., Kato S., Shimizu N., Otoki Y., Ito J., Sakaino M., Sano T., Imagi J, & Nakagawa K. (2022). Elucidation of Olive Oil Oxidation Mechanisms by Analysis of Triacylglycerol Hydroperoxide Isomers Using LC-MS/MS. Molecules, 27(16), 5282.

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Quantification of 25-Hydroxyvitamin D

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The concentration of 25OHD was determined by the immunochemiluminescence method using the DiaSorin Liaison XL analyzer (DiaSorin S.p.A., Saluggia VC, Italy; Chemiluminescent immunoassay (CLIA)). Samples were furtherly realized for 25OHD quantification using liquid chromatography coupled with tandem mass spectrometry (QTRAP^®4500 (Sciex, Framingham, MA, USA) coupled with ExionLC HPLC system (Sciex, Framingham, MA, USA); Liquid Chromatography – Mass Spectrometry (LC-MS) method), as published previously [21 (link)].

Śledzińska K., Landowski P., Żmijewski M.A., Kamińska B., Kowalski K, & Liberek A. (2022). Diet, Sun, Physical Activity and Vitamin D Status in Children with Inflammatory Bowel Disease. Nutrients, 14(5), 1029.

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