Cell supernatant was collected, and the levels of inflammatory factors IL-18 (EMC011.96; NeoBioscience, Shenzhen, Guangdong, China), IL-1β (EMC001b.96.2; NeoBioscience), IL-6 (ab178013, Abcam) and IL-10 (ab185986, Abcam) were measured using ELISA kits in accordance with the manufacturer’s instruction for each specific experimental steps.
Emc011
Manufactured by Neobioscience
Sourced in China
EMC011.96 is a laboratory equipment designed for scientific research purposes. It serves as a core function for various applications in the field of bioscience. The detailed specifications and intended use of this product are not available.
Automatically generated - may contain errors
Lab products found in correlation
Emc001b, by Neobioscience (2 mentions)
Ab178013, by Abcam (1 mentions)
Ab185986, by Abcam (1 mentions)
Cfse cell division tracker kit, by BioLegend (1 mentions)
Granulocyte macrophage colony stimulating factor, by Sino Biological (1 mentions)
Interleukin 4 (il 4), by Sino Biological (1 mentions)
Anti f4 80 microbeads, by Miltenyi Biotec (1 mentions)
4 protocols using emc011
1
Inflammatory Cytokine Measurement by ELISA
2
Quantification of IL-1β and IL-18 in BV-2 cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentrations of IL-1β and IL-18 in the supernatants of BV-2 cells exposed to different treatments were quantified by using IL-1β and IL-18 ELISA kits, respectively (IL-1β cat. EMC001b.96.2, IL-18 cat. EMC011.96; NeoBioscience, Shenzhen, China) according to the manufacturer's instructions.
3
Quantification of Neuroinflammatory Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
4
Evaluating Phagocytic Capacity of Dendritic Cells and Macrophages
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bone marrow-derived dendritic cells (BMDCs) were isolated from the bone marrow after 8 ~ 10 days of differentiation with granulocyte-macrophage colony-stimulating factor (20 ng/mL, Sino Biological) and IL-4 (10 ng/mL, Sino Biological). Macrophages were purified from tumor tissues with Anti-F4/80 MicroBeads (130-110-443, Miltenyi Biotech Inc.). BMDCs and macrophages were stained with the CFSE Cell Division Tracker Kit (423801, BioLegend). CT26-RFP cells were treated with drugs as indicated for 4 h and then added to the CFSE-labeled BMDC or macrophage culture at a 1:1 ratio. For flow cytometry, the cells were harvested and tested on a NovoCyte flow cytometer. BMDCs and macrophages that phagocytosed CT26 cells were CFSE and RFP-double-positive. For immunofluorescence, cells were fixed in 4% paraformaldehyde. Cell nuclei were stained with DAPI. The concentrations of IL-1β (EMC001b, Neobioscience), IL-12 (EMC006, Neobioscience), IL-18 (EMC011, Neobioscience), and CXCL9 (EK0733, BOSTER) in the supernatant after 24 h of co-culture were quantified using the ELISA Kit.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!