Isolated cardiomyocytes were simultaneously labeled with anti-RyR2 antibody (1:50, ab196355 Abcam) and anti-AGE (MAGE, 1:50 STA-011 Cell Biolabs). The secondary antibodies were conjugated to proximity ligation assay (PLA) oligo probes (PLA probe rabbit PLUS [DUO 92002] and PLA probe mouse MINUS [DUO 92004], Sigma) and were hybridized for 1h, 37°C. z-planes were acquired with a spectral confocal microscope (FluoView-1000, Olympus) and the number of positive cross-reactivity spots was quantified in backgroundsubtracted images (Image J).
Ab196355
Manufactured by Abcam
Ab196355 is a primary antibody. It is used for the detection of target proteins in various applications, such as Western blotting, immunohistochemistry, and ELISA. The antibody is specific to the target protein and can be used to identify and quantify its presence in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ab196355
1
Cardiomyocyte RyR2-AGE Colocalization
2
Quantification of Glycated RyR2 in SR
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Glycation of RyR2 was quantified in SR vesicles by sandwich ELISA. RyR2 was membrane-solubilized with 0.1% CHAPS (3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate) and added to previously coated wells with 1 µg of anti-RyR2 antibody (ab196355 Abcam) for immunocapture. 25 (link) Immunodetection of glycated RyR2 was performed using a biotinylated anti-AGE antibody (see Methods in the online-only Data Supplement).
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