Blue sepharose

GSH, Cys, CysGly, ȖGluCys, BSA, SDS, 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB), trans-2nonenal, NADP + , NADPH, 9,10-PQ, and sequencing-grade trypsinwere purchased from Sigma Aldrich (St. Louis, MO, USA). 4-Oxo-nonenal (ONE), prostaglandin B 1 (PGB 1 ) and 4-hydroxy-2nonenal mercapturic acid were purchased from Cayman Chemicals (Ann Arbor, MI, USA) Whatman DEAE-cellulose (DE-52) and Sephacryl S200 were purchased from GE Healthcare (Little Chalfont, UK). Blue Sepharose and Bradford reagent were purchased from Bio-Rad (Hercules, CA, USA). YM10 membranes (10kDa cut-off) were purchased from Amicon Millipore (Darmstadt, Germany). RPMI 1640, penicillin, streptomycin, glutamine and foetal bovine serum (FBS) were obtained from Lonza (Basel, Switzerland). Dialysis tubing (10kDa cut-off) was purchased from Spectrum Laboratories Inc (Rancho Dominguez, CA, USA). All inorganic chemicals were of reagent grade from BDH (VWR international ltd, Poole Dorset, UK). All solvents were HPLCgrade from J.T. Baker Chemicals (USA).

2.1 Materials. 9,10-phenanthrenquinone (PQ), bovine serum albumin, dimethyl sulfoxide (DMSO), D,L-dithiotreitol (DTT), reduced glutathione (GSH), hexanal, NADP + , nonanal, propanal, protease inhibitors cocktail, sodium dodecyl sulphate (SDS), trans-2-hexenal, trans-2-nonenal, trans-2propenal (acrolein) were purchased from Sigma-Aldrich (St. Louis, MO, USA). HHE was purchased from Cayman Chemicals (AnnArbor, MI, USA). 4(R)-HNE was purchased from Avanti Polar Lipids (Alabaster, AL, USA). Whatman DEAE-cellulose (DE-52) and Sephacryl S200 were purchased from GE Healthcare (Little Chalfont, UK). Blue Sepharose and Bradford reagent were purchased from Bio-Rad (Hercules, CA, USA). YM10 membranes (10 kDa cut-off) were purchased from Amicon Millipore (Darmstadt, Germany). Dialysis tubing (10 kDa cut-off) was purchased from Spectrum Laboratories (Rancho Dominguez, CA, USA). All inorganic chemicals were of reagent grade from BDH (VWR International, Poole, Dorset, UK). All solvents were HPLC grade from J.T. Baker Chemicals (Center Valley, PA, USA). NADPH was from Carbosynth (Compton, England).