For imaging of FRET, cells were subjected to time-lapse, dualemission microscopy with an interval of every 30 sec. Beginning at 10 min, cells were treated with 2 μM imatinib. To represent FRET efficacy, the FRET/CFP emission ratio was calculated as the quotient of background-subtracted FRET and CFP images and normalized to that at time 0. In Fig. 3, FRET/CFP ratio images are shown in intensity-modulated display mode, in which eight colors from red to blue represent the normalized emission ratios, with the intensity of each color indicating the mean intensity of FRET.
Glass bottom dishes
Glass-bottom dishes are laboratory equipment used for cell culture and microscopy applications. They provide a transparent glass surface that allows for direct observation and imaging of cells or other samples under a microscope.
Lab products found in correlation
4 protocols using glass bottom dishes
Cellular FRET Dynamics with Imatinib Treatment
For imaging of FRET, cells were subjected to time-lapse, dualemission microscopy with an interval of every 30 sec. Beginning at 10 min, cells were treated with 2 μM imatinib. To represent FRET efficacy, the FRET/CFP emission ratio was calculated as the quotient of background-subtracted FRET and CFP images and normalized to that at time 0. In Fig. 3, FRET/CFP ratio images are shown in intensity-modulated display mode, in which eight colors from red to blue represent the normalized emission ratios, with the intensity of each color indicating the mean intensity of FRET.
Visualizing Mitochondrial Morphology in H69 Cells
Mammalian Cell Expression Vectors and Reagents
Wound Healing Assay with Dynamin Inhibitors
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