Recombinant human ugt isoforms

4-Methylumbelliferone (4-MU), 4-methylumbelliferone-β-D-glucuronide (4-MUG), Tris-HCl, 7-hydroxycoumarin, trifluoperazine (TFP, purity≥ 99%), and uridine-5′-diphosphoglucuronic acid trisodium salt (UDPGA) were purchased from Sigma-Aldrich (St Louis, MO). Recombinant human UGT isoforms (UGT1A1, UGT1A3, UGT1A4, UGT1A6, UGT1A7, UGT1A8, UGT1A9, UGT1A10, UGT2B4, and UGT2B7) expressed in baculovirus-infected insect cells were obtained from BD Gentest Corp. (Woburn, MA, USA). Compounds schisandrin A, schisandrol A, schisandrin, schisandrol B, schisandrin C, schisantherin A, schisanhenol, gomisin J, gomisin D, and gomisin G were purchased from Sichuan Weikeqi Company (Chengdu, Sichuan, China). The purity of these compounds was above 95 %. All other reagents were of HPLC grade or of the highest grade commercially available.

PAEs were purchased from J&K Chemical (Beijing, China). Recombinant human UGT isoforms (UGT1A1, UGT1A3, UGT1A6, UGT1A7, UGT1A8, UGT1A9, UGT1A10, UGT2B4, UGT-2B7, UGT2B15 and UGT2B17) expressed in baculovirus-infected insect cells were purchased from BD Gentest Corp. (Woburn, MA, US). 4-Methylumbelliferone (4-MU), UDPGA (Trisodium salt), Tris-HCl, MgCl₂ and 7-hydroxycoumarin were purchased from Sigma-Aldrich (St. Louis, MO, US). Millipore Elix 5 UV and Milli-Q Gradient Ultra-Pure Water System was used to make ultra-pure water. All other reagents were of high-performance liquid chromatography (HPLC) grade or of the highest grade commercially available.