Atcc ccl 131tm

Neuro-2a (N2a), a clonal cell line originally derived from mouse neuroblastoma, was purchased from the Bioresources Collection and Research Center ([BCRC-60026, https://catalog.bcrc.firdi.org.tw/BcrcContent?bid=60026 (accessed on 10 Jan 2022)], Hsinchu, Taiwan). Neuro-2a cells, originally derived from the American Type Culture Collection (ATCC^® [CCL-131^TM]; Manassas, VA, USA), has been used as electrically excitable cells in many studies of electrophysiology and pharmacology [11 (link),33 (link),34 (link),35 (link),36 (link)]. Cells were cultured in DMEM supplemented with 10% (v/v) heat-inactivated fetal bovine serum, 2 mM L-glutamine, 1.5 g/liter sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate in a humidified atmosphere of CO₂/air (1:19) at 37 °C [21 (link),37 (link),38 (link)]. Subcultures were made by trypsinization (0.025% trypsin solution [HyClone^TM] containing 0.01% sodium, N,N,-diethyldithiocarbamate and EDTA). Electrophysiological measurements were commonly conducted when cells reached 50–70% confluence (usually 5–7 days) [21 (link)].

Neuro-2a (N2a), a clonal cell line originally derived from mouse neuroblastoma, was acquired from the Bioresources Collection and Research Center ([BCRC-60026, https://catalog.bcrc.firdi.org.tw/BcrcContent?bid=60026], (accessed on 10 Jan 2022), Hsinchu, Taiwan). This cell line, originally derived from the American Type Culture Collection (ATCC^® [CCL-131^TM]; Manassas, VA, USA), has been established as a model of electrically excitable cells in the studies of electrophysiology and pharmacology [63 (link),64 (link),66 (link),77 (link),78 (link),79 (link)]. Cells were maintained in DMEM supplemented with 10% (v/v) heat-inactivated fetal bovine serum, 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate in a humidified atmosphere of CO₂/air (1:19) at 37 °C [77 (link),80 (link)]. The subcultures were made by trypsinization (0.025% trypsin solution [HyClone^TM] containing 0.01% sodium N,N-diethyldithiocarbamate and EDTA). The measurements were performed five or six days after cells were cultured up to 60–80% confluence.