All tissue specimens were immersed in 2% formaldehyde and 0.002% picric acid fixation solution followed by 30% sucrose solution for fixation and dehydration. The tissues were subsequently frozen into optimal cutting temperature compound (OCT, Sakura Finetek, Torrance, CA) and sliced into 5 µm-thickness sections. The tissue sections were incubated with primary antibodies: anti-neuronal nitric oxide synthase (nNOS) (Santa Cruz Biotechnologies, Santa Cruz, CA), anti-neurofilament (NF, Abcam, Cambridge, MA) antibody, and anti-s100 antibody (Abcam, Cambridge, MA). Alexa-488 conjugate antibody (Invitrogen, Waltham, MA) was used for secondary antibody staining. Alexa-488 or 594 conjugated phalloidin (Pha, Invitrogen, Waltham, MA) was used for vessel expression. Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, Waltham, MA).
Anti neurofilament nf
Manufactured by Abcam
Sourced in Canada, United Kingdom
Anti-neurofilament (NF) is a lab equipment product that is used to detect and measure neurofilaments, which are structural proteins found in neurons. Neurofilaments are important for maintaining the shape and function of nerve cells. The anti-neurofilament product can be used in various research applications, such as studying neurological diseases and disorders.
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Lab products found in correlation
Anti s100 antibody, by Abcam (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Anti gfap, by Abcam (1 mentions)
Anti laminin, by Abcam (1 mentions)
Anti tuj1, by Abcam (1 mentions)
Anti tyrosine hydroxylase, by Abcam (1 mentions)
Anti cd68, by Abcam (1 mentions)
Fluorescence microscope, by Olympus (1 mentions)
2 protocols using anti neurofilament nf
1
Immunohistochemical Analysis of Neural Markers
2
Spinal Cord Tissue Immunostaining
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Spinal cord tissue sections were blocked and incubated with the following primary antibodies: anti-CD68 (1:100), anti-C–C motif receptor (CCR)7 (1:500), anti-arginase (Arg)-1 (1:1000), anti-Tuj-1 (1 µg/ml), anti-chondroitin sulfate (CS-56) (2.5 µg/ml), anti-laminin (1:50), anti-GFAP (1:1000), anti-tyrosine hydroxylase (TH) (1:100) and anti-neurofilament (NF) (1:500) (Abcam, Cambridge, UK). The following day, the samples were stained with the respective secondary antibodies (Cyanine 3, 1:200; Biosharp, Alexa Fluor® 488, 1:200; Biosharp). Finally, the sections were sealed and images were obtained using a fluorescence microscope (Olympus, Tokyo, Japan).
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