The largest database of trusted experimental protocols

Bnip3

Manufactured by Genechem
Sourced in China

BNIP3 is a gene that encodes a protein involved in the regulation of programmed cell death, or apoptosis. The BNIP3 protein plays a role in mitochondrial function and can promote cell death under certain conditions. This product is a research tool used to study the biological functions of the BNIP3 gene and protein.

Automatically generated - may contain errors

2 protocols using bnip3

1

Stable Cell Line Generation for BNIP3 Knockdown

Check if the same lab product or an alternative is used in the 5 most similar protocols
mRFP-LC3 (21075), tfLC3 (21074), and LAMP1-mGFP(34831) were obtained from Addgene (Cambridge, MA, USA). EGFP-LC3, BNIP3, and SNAP29 plasmids were constructed by Gene Chem Co. Ltd (Shanghai, China). Cells were transfected with plasmids using Lipofectamine 3000 Transfection Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. After 24 h incubation, the transfection mixture was removed and replaced with fresh complete medium. The target sequence of BNIP3 shRNA (5′-ACTGCACTTCAGCAATAAT-3′) was purchased Gene Chem Co. Ltd. To establish BNIP3 knockdown stable cell lines, 293FT cells were co-transfected with lentiviral packaging vectors pLP1, pLP2, and pLP/VSVG (Invitrogen, K4975) along with shBNIP3 or shCon plasmid using Lipofectamine 3000 (Invitrogen, L3000015) according to the manufacturer’s protocols. Forty-eight hours later, supernatant containing the lentivirus was harvested and infected with MCF-7 cells. Cells were subsequently selected with 4 μg/mL puromycin (Sigma, P9620) to establish stable cell lines.
+ Open protocol
+ Expand
2

Osteoclast Differentiation Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Osteoclast precursors were seeded into six well plates at 4 × 105 cells/well. The cells were transfected with lentiviral particles for overexpression of BNIP3 or E2F1 (Genechem, Shanghai, China) using polybrene in the presence of 30 ng/mL M-CSF. After 48 h and washing three times to stop the infection, cells were incubated with vehicle or metformin in the presence of 30 ng/mL M‐CSF and 50 ng/mL RANKL.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!