α h3k36me3 ab9050

Manufactured by Abcam

Sourced in Japan

α-H3K36me3(ab9050) is a primary antibody that specifically recognizes the tri-methylation of lysine 36 on histone H3. This post-translational modification is associated with active transcription.

Automatically generated - may contain errors

Lab products found in correlation

Phage ef1 dcas9 krab, by Addgene (1 mentions) Imagequant tl software, by GE Healthcare (1 mentions) α gfp, by Roche (1 mentions) αflag, by Fujifilm (1 mentions) Trichloroacetic acid, by Merck Group (1 mentions)

Close spelling variants of this product

H3K36me3 (ab9050) α-H3K36me3 Ab9050 H3K36me3

2 protocols using α h3k36me3 ab9050

ChIP-seq analysis using dCas9-KRAB

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The pHAGE EF1-dCas9-KRAB (Addgene plasmid 50919) and pLKO.1-puro U6 sgRNA BfuAI stuffer lentiviral plasmids were developed by Rene Maehr and Scot Wolfe (Addgene plasmid 50920).⁴⁰ (link) The ChIP-grade antibodies used in this study, α-H3K9me3 (ab8898), α-H3K36me3 (ab9050), and α-histone H3 (ab1791), were purchased from Abcam (Cambridge, MA).

Himeda C.L., Jones T.I., Virbasius C.M., Zhu L.J., Green M.R, & Jones P.L. (2018). Identification of Epigenetic Regulators of DUX4-fl for Targeted Therapy of Facioscapulohumeral Muscular Dystrophy. Molecular Therapy, 26(7), 1797-1807.

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Protein Extraction and Western Blot Analysis

Cited 2 times

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Asynchronous cells were collected, and total protein was extracted using 20% trichloroacetic acid (Sigma-Aldrich) (71 (link),76 (link)). Western blotting and immunostaining were carried out according to previously published procedures (24 (link),52 (link)). The following antibodies were employed: αHA (11583816001, Roche Diagnostics, Basel, Switzerland), αGFP (1181446001, Roche Diagnostics), αFLAG (014–22383, Wako Chemicals, Osaka, Japan), αSer5P-PolII (H14, Covance, Princeton, NJ, USA), αTAT1 (56 (link)), αH3K36me2 (MABI0332, MAB Institute Inc., Kanagawa, Japan), αH3K36me3 (ab9050, Abcam, Cambridge, UK) and αCdc2 antibodies (sc-53, Santa Cruz Biotechnology, Dallas, TX, USA). Chemiluminescence signal was imaged using an ImageQuant LAS 4000 imager, with band intensities quantified using ImageQuant TL software (GE Healthcare).

Lim K.K., Lam U.T., Li Y., Zeng Y.B., Yang H, & Chen E.S. (2024). Set2 regulates Ccp1 and Swc2 to ensure centromeric stability by retargeting CENP-A. Nucleic Acids Research, 52(8), 4198-4214.

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