The paraffin embedded brains were prepared on the third day after ICH with the method described above. For the primary cortical neurons, the cells were seeded into 24-well culture plate at 2 × 105 cells/well for immunofluorescent staining. Tissue sections and cells were incubated overnight at 4 °C with rabbit anti-MAP2 monoclonal antibody (1:2000, Abcam, Cambridge, MA, USA), rabbit anti-NeuN monoclonal antibody (1:100, Abcam, Cambridge, MA, USA), rabbit anti-AIFM2 polyclonal antibody (1:100, Affinity Biosciences, OH, USA), mouse anti-GPX4 monoclonal antibody (1:50, Santa Cruz Biotechnology, CA, USA), or rabbit anti-FACL4 monoclonal antibody (1:100, Abcam, Cambridge, MA, USA). After PBS rinsing, the samples were incubated in the corresponding Alexa Fluor-conjugated secondary antibody (1:500, Abbkine, USA) for 1 h in the dark at room temperature. The anti-fluorescence quenching mounting medium containing DAPI (Solarbio, Beijing, China) was added to the sample area. Then all sections and cells were observed under fluorescence microscope and the positive cells or mean fluorescent intensity in 4 fields per section/wells were counted with Image Pro-Plus 6.0 software.
Rabbit anti map2 monoclonal antibody
Manufactured by Abcam
Sourced in United States, China
Rabbit anti-MAP2 monoclonal antibody is a primary antibody that specifically recognizes the microtubule-associated protein 2 (MAP2) in various species. MAP2 is a cytoskeletal protein involved in the stabilization and regulation of microtubule assembly. This antibody can be used for the detection and analysis of MAP2 expression in different cell and tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti aifm2 polyclonal antibody, by Affinity Biosciences (1 mentions)
Mouse anti gpx4 monoclonal antibody, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti facl4 monoclonal antibody, by Abcam (1 mentions)
Inverted phase contrast fluorescence microscope, by Nikon (1 mentions)
Triton x 100, by Merck Group (1 mentions)
2 protocols using rabbit anti map2 monoclonal antibody
1
Immunofluorescent Staining of ICH Brain Samples
2
Immunocytochemical Characterization of NSCs
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NSCs were identified using standard immunocytochemical staining for Nestin, while differentiated NSCs were identified by staining for GFAP, GALC, NF-H and MAP2, which are markers of astrocytes, oligodendrocytes and neurons, respectively. Cells were fixed with paraformaldehyde (4%, w/v) in PBS and then permeabilized with 0.1% Triton X-100 (Sigma) at room temperature for 10 min. After being blocked with 5% BSA for 1 h, the cells were washed and incubated with primary rabbit anti-Nestin polyclonal antibody (1:500 dilution; Bioss, Beijing, China), primary rabbit anti-GFAP polyclonal antibody (1:200 dilution; Bioss), primary rabbit anti-GALC polyclonal antibody (1:200 dilution; Absin, Shanghai, China), primary rabbit anti-NF-H polyclonal antibody (1:200 dilution; Bioss), and rabbit anti-MAP2 monoclonal antibody (1:500 dilution; Abcam) at 4°C overnight. After three washes, the cells were incubated with a goat anti-rabbit IgG-Cy3 secondary antibody (1:200 dilution; Absin) at room temperature for 2 h. The nuclei were counterstained with DAPI at room temperature for 10 min. The samples were visualized with a fluorescence phase contrast inverted microscope (Nikon, Tokyo, Japan).
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