Vs 110 scanner

Manufactured by Olympus

The VS-110 is a high-performance slide scanner that captures digital images of microscope slides. It features a high-resolution camera and advanced optics to provide detailed, high-quality scans of biological samples.

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Lab products found in correlation

Visiomorph dp, by Visiopharm (1 mentions) Triton x 100, by Merck Group (1 mentions) Goat serum, by Thermo Fisher Scientific (1 mentions) Jbw301, by Merck Group (1 mentions) Ab680, by MedChemExpress (1 mentions)

2 protocols using vs 110 scanner

Co-immunofluorescence Profiling of HGSC

Cited 1 time

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Co-immunofluorescence (IF) was performed in a previously described cohort of 200 HGSC on tissue microarray (TMA)9 (link) and in a previously described cohort of 1000 HGSC (from the Canadian Ovarian Experimental Unified Resource)25 (link) on TMA (online supplemental table S1). Only cases that did not receive neoadjuvant chemotherapy were included for analyses. Slides were deparaffinized, rehydrated, demasked using a citrate buffer and incubated for 30 min with a protein blocking solution. Primary antibodies were co-incubated on the slides overnight at 4°C and secondary antibodies were incubated for 2 hours at room temperature (online supplemental table S2). Slides were then mounted with 4′,6-diamidino-2-phenylindole (DAPI). Sections were imaged at 20X magnification on VS-110 scanner (Olympus) and super images were imported into Visiomorph DP (Visiopharm). CK8-18 positivity was used to identify the epithelial area and where indicated α smooth muscle actin (SMA) positivity was used to identify the activated stromal area. Cores with epithelial or stromal areas representing less than 5% of the total tissue area were eliminated from survival analyses. Recommendations for Tumor Marker Prognostic Studies criteria were followed.26 (link)

Bareche Y., Pommey S., Carneiro M., Buisseret L., Cousineau I., Thebault P., Chrobak P., Communal L., Allard D., Robson S.C., Mes-Masson A.M., Provencher D., Lapointe R, & Stagg J. (2021). High-dimensional analysis of the adenosine pathway in high-grade serous ovarian cancer. Journal for Immunotherapy of Cancer, 9(3), e001965.

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Quantitative DNA Damage Analysis

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Cell lines were seeded on glass-cover slips, allowed to attach for 24 hours, and then treated for 48 hours with gemcitabine, radiation (1Gy using Gammacell^® 3000 irradiator Elan), BAY 60–6583 (1μM; Tocris, cat #4472). or AB680 (0.1μM; MedChem Express, cat #2105904–82-1). Cells were fixed 10 minutes with 10% formalin and permeabilized 15 minutes with PBS, 0.25% Triton-X100 (Sigma). Unspecific binding sites were blocked for 1 hour using PBS, 1% BSA, 4% goat serum (ThermoFisher). Primary antibody against γH2AX (Milipore, JBW301, 1/2000) was incubated overnight at 4°C. Goat anti-mouse antibody coupled with Alexa Fluor-647 was incubated for 1 hour at room temperature (1/400 dilution), followed by DAPI staining. Cover slips were mounted on glass slides and imaged at 40X magnification with a VS-110 scanner (Olympus). Images were then analyzed with Visiomorph DP image analysis software (VIS, Visiopharm) allowing automated nucleus and γH2AX foci count following a sequential process. DAPI counterstaining was used for nuclei identification, and for each nucleus, the number of γH2AX foci was calculated. For each replicate, at least 20 nuceli are analyzed and each condition was tested in triplicate.

Jacoberger-Foissac C., Cousineau I., Bareche Y., Allard D., Chrobak P., Allard B., Pommey S., Messaoudi N., McNicoll Y., Soucy G., Koseoglu S., Masia R., Lake A.C., Seo H., Eeles C.B., Rohatgi N., Robson S.C., Turcotte S., Haibe-Kains B, & Stagg J. (2023). CD73 inhibits cGAS-STING and cooperates with CD39 to promote pancreatic cancer. Cancer immunology research, 11(1), 56-71.

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