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Superoxide dismutase sod

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Superoxide dismutase (SOD) is an enzyme that catalyzes the dismutation of superoxide radicals into oxygen and hydrogen peroxide. It is a crucial antioxidant defense mechanism in aerobic organisms.

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Lab products found in correlation

Tnf α, by Beyotime (2 mentions) Malondialdehyde mda, by Solarbio (2 mentions) T aoc, by Solarbio (2 mentions) Interleukin 6 (il 6), by Abcam (2 mentions) Mcp 1, by Abcam (2 mentions) Lipopolysaccharides (lps), by Cusabio (2 mentions) Sybr premix ex taq 2, by Takara Bio (1 mentions) Rnaiso plus, by Takara Bio (1 mentions) Primescript rt reagent kit with gdna eraser, by Takara Bio (1 mentions) Cell counting kit 8 (cck8), by Beyotime (1 mentions) Gapdh, by Proteintech (1 mentions) Lipofectamine 2000, by GenePharma (1 mentions) 2 7 dichlorofluorescin diacetate, by Solarbio (1 mentions) Cerulein, by Merck Group (1 mentions) Resveratrol, by Merck Group (1 mentions) Indigo carmine, by Merck Group (1 mentions) Veratryl alcohol, by Merck Group (1 mentions) Remazol brilliant blue r, by Merck Group (1 mentions) N acetyl l cysteine nac, by Merck Group (1 mentions) 2 7 dichlorodihydrofluorescein diacetate dcfh da, by Merck Group (1 mentions)

6 protocols using superoxide dismutase sod

1

Antioxidant Assays and Gene Regulation

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Cell Counting Kit-8 was obtained from Beyotime (Shanghai, China). Catechin (C), epicatechin (EC), epicatechin gallate (ECG), procyanidin B1 (B1), procyanidin B2 (B2), procyanidin B3 (B3), procyanidin B4 (B4), procyanidin B1-3-O-gallate (B1-G), procyanidin B2-3-O-gallate (B2-G), and procyanidin C1 (C1) were obtained from Caoyuankang Biotechnology. 2′,7′-Dichlorofluorescin diacetate, malondialdehyde (MDA), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) diagnostic kits were obtained from Solarbio (Beijing, China). Nuclear factor-erythroid 2-related factor 2-siRNA (Nrf2-siRNA), control-siRNA, and Lipofectamine 2000 were obtained from GenePharma (Shanghai, China). The primary antibodies, Nrf2, heme oxygenase 1 (HO-1), NAD(P)H: quinone oxidoreductase 1 (NQO1), Lamin B, and GAPDH, as well as corresponding secondary antibodies, were obtained from Proteintech (Wuhan, China). RNAiso Plus, PrimeScript RT reagent Kit with gDNA Eraser, and SYBR Premix Ex Taq II were purchased from Takara (Takara, Shiga, Japan).
Chen J., Chen Y., Zheng Y., Zhao J., Yu H, & Zhu J. (2022). Relationship between Neuroprotective Effects and Structure of Procyanidins. Molecules, 27(7), 2308.
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2

Resveratrol Modulates Inflammation and Oxidation

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Resveratrol (≥99%, Sigma, USA), sodium carboxymethyl cellulose (UNIVAL, Germany), cerulein (Sigma, USA), total cholesterol (TC) and total triglyceride (TG) content assay kits (Solarbio, China), LPS (cusabio, China), MCP-1 (Abcam, USA), TNF-α (Beyotime, China), IL-6 (Abcam, USA) malondialdehyde (MDA) (Solarbio, China), superoxide dismutase (SOD) (Solarbio, China), and total antioxidant capacity (T-AOC) (Solarbio, China) ELISA kits.
Zhang X., Yang G., Chen Y., Mu Z., Zhou H, & Zhang L. (2022). Resveratrol pre-treatment alleviated caerulein-induced acute pancreatitis in high-fat diet-feeding mice via suppressing the NF-κB proinflammatory signaling and improving the gut microbiota. BMC Complementary Medicine and Therapies, 22, 189.
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3

Characterization of Irpex lacteus CD2 Enzyme Activity

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Irpex lacteus CD2 was isolated from Shennong Nature Reserve (Hubei province, China) and preserved in the Institute of Environment & Resource Microbiology, Huazhong University of Science & Technology, Wuhan, China. I. lacteus CD2 was maintained at 4 °C on potato-dextrose agar (PDA) plate. Substrates 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,6-dimethylphenol (DMP), veratryl alcohol (VA), guaiacol, and dyes with various structures including remazol brilliant violet 5R (RBV5R), reactive black 5 (RB5), remazol brilliant blue R (RBBR), methyl green (MG), and indigo carmine (IC) were purchased from Sigma-Aldrich (St. Louis, MO). The superoxide dismutase (SOD) was purchased from Solarbio (Beijing, China). The structures for the substrates, synthetic dyes, and non-phenolic lignin model compound are listed in Table 2.
Qin X., Sun X., Huang H., Bai Y., Wang Y., Luo H., Yao B., Zhang X, & Su X. (2017). Oxidation of a non-phenolic lignin model compound by two Irpex lacteus manganese peroxidases: evidence for implication of carboxylate and radicals. Biotechnology for Biofuels, 10, 103.
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4

Investigating CDK4/6 Inhibitor Combination Therapy

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PD 0332991(CDK4/6 inhibitor; #HY-50767, CAS Number: 571190-30-2) was purchased from MedChemExpress LLC (Shanghai, China). DDP (cisplatin; #BP809, CAS Number: 15663-27-1), N-Acetyl-L-cysteine (NAC; #A7250, CAS Number: 616-91-1), 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA; #287810, CAS Number: 4091-99-0) and Dimethyl sulfoxide (DMSO; #D2650, CAS Number: 67-68-5) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cell cycle assay kits (#KGA512) were purchased from KeyGEN BioTECH (Jiangsu, China). Superoxide Dismutase (SOD; # S8410, CAS Number: 9054-89-1) was purchased from Beijing Solarbio Science & Technology Co. (Beijing, China). Catalase (#MB3116, CAS Number: 9001-05-2) and Glutathione (GSH; # 571190-30-2, CAS Number: 70-18-8) were purchased from Dalian Melun Biotechnology Co. (Dalian, China). MK2206 (Akt inhibitor; # S1078, CAS Number: 1032350-13-2) was purchased from Selleck Chemicals (Shanghai, China). A549-myr-Akt cells were a kind gift from Dr Junchao Cai, Sun Yat-sen University Zhongshan School of Medicine [36 (link)].
Wang L., Huang Y., Huang C.H., Yu J.C., Zheng Y.C., Chen Y., She Z.G, & Yuan J. (2020). A Marine Alkaloid, Ascomylactam A, Suppresses Lung Tumorigenesis via Inducing Cell Cycle G1/S Arrest through ROS/Akt/Rb Pathway. Marine Drugs, 18(10), 494.
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5

2'-FL Antioxidant Evaluation Protocol

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2′-FL (purity ≥ 98%, cat# GY1141) was purchased from HuicH Technology Co., Ltd. (Shanghai, Beijing, China). Glutathione (GSH, cat: BC1170), malondialdehyde (MDA, cat: BC0020), total antioxidant capacity (T-AOC, cat: BC1310), and superoxide dismutase (SOD, cat: BC0170) were bought from Solarbio Life Science (Beijing, China).
Yao Q., Gao Y., Fan L., Wang J, & Zheng N. (2022). 2′-Fucosyllactose Remits Colitis-Induced Liver Oxygen Stress through the Gut–Liver–Metabolites Axis. Nutrients, 14(19), 4186.
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6

Proinflammatory and Antioxidant Markers in Mice

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Blood was collected from mice in each group and centrifuged at 15000 rpm for 10 min to obtain the serum. In the first part of experiments, the levels of proinflammatory cytokines including LPS (cusabio, China), MCP-1 (Abcam, USA), TNF-α (Beyotime, China), and IL-6 (Abcam) were determined by ELISA according to the manufacturer’s instructions. In the second part of experiments, the levels of malondialdehyde (MDA) (Solarbio, Beijing, China), superoxide dismutase (SOD) (Solarbio, Beijing, China), and total antioxidant capacity (T-AOC) (Solarbio, Beijing, China) were measured by ELISA according to the manufacturer’s instructions. The OD value of each well was immediately read at 450 nm.
Zhang X., Yang G., Chen Y., Mu Z., Zhou H, & Zhang L. (2022). Resveratrol pre-treatment alleviated caerulein-induced acute pancreatitis in high-fat diet-feeding mice via suppressing the NF-κB proinflammatory signaling and improving the gut microbiota. BMC Complementary Medicine and Therapies, 22, 189.
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