Blood samples were collected from NSG mice bearing subcutaneous PDX tumors at the time of sacrifice. The PDX lines were maintained at vivaria in the University of Washington and FHCC. The blood was processed following methods described for human plasma DNA processing for subsequent DNA isolation. Blood was collected in Sarstedt Micro sample tube K3 EDTA tubes and processed within 4 hours. All blood samples were sequentially double spun, first at 2500g for 10 minutes followed by a 16000g centrifugation of the plasma fraction for 10 minutes at room temperature. For each PDX line, 4–8 mouse plasma samples were pooled. Processed plasma samples were preserved in clean, screw-capped cryo-microfuge tubes and stored at −80°C prior to cfDNA isolation.
Micro sample tube k3 edta tubes
Manufactured by Sarstedt
The Micro sample tube K3 EDTA tubes are a type of laboratory equipment used for the collection and storage of small biological samples. They are designed to contain the anticoagulant K3 EDTA, which helps prevent the samples from clotting. The tubes are suitable for various applications in clinical and research settings that require the preservation of blood and other liquid samples.
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Lab products found in correlation
2 protocols using micro sample tube k3 edta tubes
1
Blood Plasma Isolation for PDX Tumors
2
Mouse Plasma Collection for cfDNA Analysis
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Blood samples were collected from NSG mice bearing subcutaneous PDX tumors at the time of sacrifice. The PDX lines were maintained at vivaria in the UW and FHCC. The blood was processed following methods described for human plasma DNA processing for subsequent DNA isolation. Blood was collected in Sarstedt Micro sample tube K3 EDTA tubes and processed within 4 hours. All blood samples were sequentially double spun, first at 2,500 × g for 10 minutes followed by a 16,000 × g centrifugation of the plasma fraction for 10 minutes at room temperature. For each PDX line, 4 to 8 mouse plasma samples were pooled. Processed plasma samples were preserved in clean, screw-capped cryo-microfuge tubes and stored at −80°C prior to cfDNA isolation.
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