Human il 2 duoset elisa kit

MTT (1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan) powder, CMFDA (5-chloromethylfluorescein diacetate) and CMRA (5-(((4-chloromethyl)benzoyl)amino) tetramethylrhodamine) cell staining dyes, was obtained from Sigma Chemical Co. (St. Louis, MO, USA). Anti-CD3 antibodies and anti-CD28 antibodies were purchased from Bioxcell (West Lebanon, NH, USA). Human IL-2 DuoSet^® ELISA kit was obtained from R&D Systems (Minneapolis, MN, USA). Staphylococcal enterotoxin E (SEE) was purchased from Toxin Technology (Sarasota, FL, USA). NE-PER Nuclear and Cytoplasmic Extraction Reagents Kit and ECL Western blotting detection reagents were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Anti-CD40L antibodies conjugated with APC was purchased from eBiosciences (San Diego, CA, USA). Anti-CD40L neutralizing antibodies were obtained from InvivoGen (San Diego, CA, USA). Anti-CD40L for western blot and anti-β-actin antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-p65, anti-PARP, anti-IκBα, anti-phosphorylated IκBα (S32), anti-phosphorylated ERK (T202/Y204), anti-ERK, anti-phosphorylated p38 (T180/Y182), anti-p38, anti-phosphorylated JNK (T183/Y185), anti-JNK, anti-phosphorylated c-Jun (S73) and anti-c-Jun antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA).