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Lipidyzer isotope labeled internal standards mixture

Manufactured by AB Sciex
Sourced in United States

The Lipidyzer isotope labeled internal standards mixture is a comprehensive set of lipid standards designed to facilitate accurate quantification of a wide range of lipid species in biological samples. The mixture contains isotopically labeled compounds that serve as internal standards, enabling precise measurement and normalization of lipid levels.

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4 protocols using lipidyzer isotope labeled internal standards mixture

1

Lipid Analysis using LC-MS

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LC-MS grade methanol, dichloromethane, and ammonium acetate were purchased from Fisher Scientific (Pittsburgh, PA) and HPLC grade 1-propanol from Sigma-Aldrich (Saint Louis, MO). Milli-Q water was obtained from an in-house Ultrapure Water System by EMD Millipore (Billerica, MA). The Lipidyzer isotope labeled internal standards mixture consisting of 54 isotopes from 13 lipid classes was purchased from Sciex (Framingham, MA).
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2

LC-MS Lipid Quantification Protocol

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Materials: LC–MS grade methanol, dichloromethane, and ammonium acetate were purchased from Fisher Scientific (Pittsburgh, PA) and HPLC grade 1-propanol was purchased from Sigma-Aldrich (Saint Louis, MO). Milli-Q water was obtained from an in-house Ultrapure Water System by EMD Millipore (Billerica, MA). The Lipidyzer isotope labeled internal standards mixture consisting of 54 isotopes from 13 lipid classes was purchased from Sciex (Framingham, MA).
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3

Lipidyzer Internal Standards LC-MS Workflow

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All LC-MS grade solvents including acetonitrile and water were purchased from Fisher Optima grade, Fisher Scientific (Pittsburgh, PA, USA). High purity formic acid (99%) LC–MS grade methanol, dichloromethane, and ammonium acetate were purchased from Fisher Scientific and HPLC grade 1-propanol, debrisoquine, and 4-nitrobenzoic acid were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Milli-Q water was obtained from an in-house Ultrapure Water System by EMD Millipore (Billerica, MA, USA). The Lipidyzer isotope-labeled internal standards mixture consisting of 54 isotopes from 13 lipid classes was purchased from SCIEX (Framingham, MA, USA).
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4

Lipid Profiling with Isotope-Labeled Standards

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Materials: Materials: LC–MS grade methanol, dichloromethane, and ammonium acetate were purchased from Fisher Scientific (Pittsburgh, PA) and HPLC grade 1-propanol was purchased from Sigma-Aldrich (Saint Louis, MO). Milli-Q water was obtained from an in-house Ultrapure Water System by EMD Millipore (Billerica, MA). The Lipidyzer isotope labeled internal standards mixture consisting of 54 isotopes from 13 lipid classes was purchased from Sciex (Framingham, MA).
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