Immunofluorescence was performed as previously described [19 (link)]. The primary antibodies and dilutions were as follows: anti-Calretinin (Millipore, AB5054, 1:500); anti-Calbindin (Millipore, AB1778, 1:250); anti-Foxg1 (Abcam, ab18259, 1:1000); anti-GFP (Abcam, ab13970, 1:1000); anti-L1 (Millipore, MAB5272, 1:500); anti-Pax6 (BioLegend, 901,301, 1:1000); and anti-Vglut2 (Millipore, MAB5504, 1:500). The secondary antibodies used were Alexa Fluro 488 donkey anti-chicken (Jackson Lab, 703–545-155, 1:500), Alexa Fluor 488 donkey anti-rabbit (Life, A21206, 1:500), Alexa Fluor 546 donkey anti-rabbit (Life, A10040, 1:500), Alexa Fluro 647 donkey anti-rabbit (Life, A31573, 1:500), Alexa Fluor 488 donkey anti-rat (Life, A21208, 1:500), CF 633 donkey anti-rat (Sigma-Aldrich, SAB4600133, 1:500) and Alexa Fluro 647 donkey anti-mouse (Invitrogen, A21236, 1:500).
Alexa fluro 488 donkey anti chicken
Manufactured by Jackson ImmunoResearch
Sourced in United States
Alexa Fluor 488 donkey anti-chicken is a secondary antibody conjugate that binds to chicken primary antibodies. It is designed for use in various immunoassay techniques, such as flow cytometry, immunohistochemistry, and Western blotting, to detect and visualize chicken antigens.
Automatically generated - may contain errors
Lab products found in correlation
Ab18259, by Abcam (2 mentions)
Alexa fluro 647 donkey anti mouse, by Thermo Fisher Scientific (2 mentions)
Ab13970, by Merck Group (1 mentions)
Ab1778, by Abcam (1 mentions)
Mab5504, by Merck Group (1 mentions)
Ab5054, by Merck Group (1 mentions)
Ab40658, by Abcam (1 mentions)
Ab18465, by Abcam (1 mentions)
Mab5364, by Merck Group (1 mentions)
Ab184337, by Abcam (1 mentions)
Ab5054, by Abcam (1 mentions)
Ab5475, by Merck Group (1 mentions)
Ab13970, by Abcam (1 mentions)
2 protocols using alexa fluro 488 donkey anti chicken
1
Comprehensive Immunofluorescence Labeling Protocol
2
Immunofluorescence Staining Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Immunofluorescence was performed as previously described [21 (link),22 (link)]. The primary antibodies and dilutions were as follows: anti-Calretinin (Millipore, AB5054, 1:500, Billerica, MA, USA); anti-Ctip2 (Abcam, ab18465, 1:2000, Cambridge, MA, USA); anti-Foxg1 (Abcam, ab18259, 1:1000, Cambridge, MA, USA); anti-GFP (Abcam, ab13970, 1:1000, Cambridge, MA, USA); anti-Lhx2 (Abcam, ab184337, 1:500, Cambridge, MA, USA); anti-P73 (Abcam, ab40658, 1:500, Cambridge, MA, USA); anti-Prox1 (Millipore, AB5475, 1:1000, Billerica, MA, USA); and anti-Reelin (Millipore, MAB5364, 1:1000, Billerica, MA, USA). The secondary antibodies used were Alexa Fluro 488 donkey anti-chicken (Jackson Lab, 703-545-155, 1:500, West Grove, PA, USA), Alexa Fluor 546 donkey anti-rabbit (Life, A10040, 1:500, Gaithersburg, MD, USA), Alexa Fluro 647 donkey anti-rabbit (Life, A31573, 1:500, Gaithersburg, MD, USA), Alexa Fluor 546 donkey anti-rat (Life, A10040, 1:500, Gaithersburg, MD, USA), CF 568 donkey anti-rat (Sigma-Aldrich, SAB4600077, 1:500, St. Louis, MO, USA), CF 633 donkey anti-rat (Sigma-Aldrich, SAB4600133, 1:500, St. Louis, MO, USA), and Alexa Fluro 647 donkey anti-mouse (Invitrogen, A21236, 1:500, Carlsbad, CA, USA).
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