Antibodies recognizing YAP1, phospho-YAP1 (Ser-127 p-YAP1)(Cell Signaling Technology, Beverly, MA, USA), matrix metalloproteinase2 (MMP2), slug, twist, vimentin, AR, NK3 Homeobox 1 (NKX3.1), Myc, connective tissue growth factor (CTGF, Santa Cruz Biotechnology, Santa Cruz, CA, USA), E-cadherin, N-cadherin (BD Biosciences, San Jose, CA, USA), phospho-AR (ser-81 p-AR)(Milipore, Billerica, MA, USA), and ß-actin, GAPDH (Sigma-Aldrich, St. Louis, MO, USA) were used for immunoblottings. Horseradish peroxidase (HRP)-conjugated donkey anti-rabbit and anti-mouse IgGs were obtained from Cell Signaling Technology. GV1001 peptide was supplied by GemVax & KAEL (Seongnam, Kyeonggi-do, South Korea). Leuprolide acetate (LA) and cetrorelix acetate (CA) were synthesized by AnyGen (Gwangju, South Korea). KT5720 (Cat# K3761), forskolin (Cat# F6886), dihydrotestosterone (Cat# A8380), flutamide (Cat# F9397), pertussis toxin (Cat# P2980) and SQ22536 (Cat# S153) were purchased from Sigma-Aldrich (St. Louis, MO, USA). KH7 (Cat# 13243, Cayman Chemical, Ann Arbor, MI, USA) and 1,2-Bis(2-aminophenoxy) ethane-N,N,N’,N’-tetraacetic acid tetrakis(acetoxymethyl ester, BAPTA/AM)(Biovision, Mountain View, CA, USA) were used as signaling inhibitors for Gα-proteins.
Horseradish peroxidase hrp conjugated donkey anti rabbit and anti mouse iggs
Manufactured by Cell Signaling Technology
Horseradish peroxidase (HRP)-conjugated donkey anti-rabbit and anti-mouse IgGs are secondary antibodies used in immunodetection techniques. They are designed to bind to primary antibodies raised in rabbits or mice, and the HRP enzyme label enables the detection of target proteins through colorimetric or chemiluminescent signals.
Automatically generated - may contain errors
Lab products found in correlation
Dihydrotestosterone, by Merck Group (1 mentions)
Flutamide, by Merck Group (1 mentions)
Sq22536, by Merck Group (1 mentions)
Forskolin, by Merck Group (1 mentions)
Pertussis toxin, by Merck Group (1 mentions)
Kt5720, by Merck Group (1 mentions)
Bapta am, by Abcam (1 mentions)
Poly adp ribose polymerase 1 parp, by Cell Signaling Technology (1 mentions)
2 protocols using horseradish peroxidase hrp conjugated donkey anti rabbit and anti mouse iggs
1
Prostate Cancer Signaling Pathway Analysis
2
Antibody-based Protein Expression Analysis
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Antibodies recognizing GnRHR (Santa Cruz Biotechnology), poly(ADP-ribose) polymerase (PARP) 1, cleaved PARP1 (cl-PARP1), cleaved caspase3 (cl-casp3), Bcl-2 (Cell Signaling Technology) and ß-actin (Sigma-Aldrich) were used for Western blotting. Horseradish peroxidase (HRP)conjugated donkey anti-rabbit and anti-mouse IgGs were obtained from Cell Signaling Technology. GV1001 peptide and fluorescein isothiocyanate (FITC)-conjugated GV1001 were supplied by GemVax and KAEL (Seongnam, Kyeonggi-do, South Korea). LA and cetrorelix acetate (CA) were purchased from AnyGen (Gwangju, South Korea).
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