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Beckman ls 9000

Manufactured by Beckman Coulter
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The Beckman LS-9000 is a liquid scintillation counter designed for high-throughput radioactive sample analysis. It measures the radioactive decay of samples by detecting the light pulses generated when the radioactive particles interact with a scintillation cocktail. The LS-9000 is capable of processing multiple samples in a rapid and efficient manner, making it suitable for a variety of applications that require the quantification of radioactive materials.

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Lab products found in correlation

Scintiverse, by Thermo Fisher Scientific (2 mentions) 3h gly sar, by Moravek Biochemicals (2 mentions)

2 protocols using beckman ls 9000

1

Caco-2 Cell Gly-Sar Uptake Inhibition

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Caco-2 cells at 10 days postseeding were incubated with 10 µmol/L Gly-Sar (9.98 µmol/L Gly-Sar and 0.02 µmol/L [3H]Gly-Sar (Moravek Biochemicals, Brea, CA, USA)), along with various concentrations (5–0.05 mmol/L) of Gem and Gem prodrugs for 30 min. After the incubation, the drug solution was removed. The cells were gently washed three times with ice-cold PBS and solubilized with 2 mL of scintillation cocktail (ScintiVerse, Fisher Scientific, St. Louis, MO, USA), and the radioactivity was determined by scintillation counting (Beckman LS-9000, Beckman Instruments, Fullerton, CA, USA). Inhibitory concentration 50 (IC50) values were determined using nonlinear data fitting (GraphPad Prism version 6, GraphPad Software, Inc., La Jolla, CA, USA).
Tsume Y., Drelich A.J., Smith D.E, & Amidon G.L. (2017). Potential Development of Tumor-Targeted Oral Anti-Cancer Prodrugs: Amino Acid and Dipeptide Monoester Prodrugs of Gemcitabine. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(8), 1322.
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2

Caco-2 Cell Gly-Sar Uptake Inhibition

Check if the same lab product or an alternative is used in the 5 most similar protocols
Caco-2 cells at 10 days postseeding were incubated with 10 µmol/L Gly-Sar (9.98 µmol/L Gly-Sar and 0.02 µmol/L [3H]Gly-Sar (Moravek Biochemicals, Brea, CA, USA)), along with various concentrations (5–0.05 mmol/L) of Gem and Gem prodrugs for 30 min. After the incubation, the drug solution was removed. The cells were gently washed three times with ice-cold PBS and solubilized with 2 mL of scintillation cocktail (ScintiVerse, Fisher Scientific, St. Louis, MO, USA), and the radioactivity was determined by scintillation counting (Beckman LS-9000, Beckman Instruments, Fullerton, CA, USA). Inhibitory concentration 50 (IC50) values were determined using nonlinear data fitting (GraphPad Prism version 6, GraphPad Software, Inc., La Jolla, CA, USA).
Tsume Y., Drelich A.J., Smith D.E, & Amidon G.L. (2017). Potential Development of Tumor-Targeted Oral Anti-Cancer Prodrugs: Amino Acid and Dipeptide Monoester Prodrugs of Gemcitabine. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(8), 1322.
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