Microseal b clear

This data set created a dilution series consisting of four 10-fold serial dilution points from 15,000 to 15 molecules, using 10 ng / μl yeast tRNA as a carrier (Roche) and created NTC samples of the same dilution. qPCR was done on a CFX 384 instrument (Bio-Rad). QPCR was performed on a CFX 384 instrument (Bio-Rad) using a 96-well pipetting robot (Tecan Freedom Evo 150). Amplification reactions were performed in 8 μl samples containing 0.4 μl forward and 0.4 μl reverse primer (5 μM each), 0.2 μl nuclease-free water, 4 μl iQ SYBR Green Supermix (Bio-Rad) and 3 μl of standard oligonucleotide. In 384-well plates (Hard-Shell 384-well microplate and Microseal B clear using an adhesive seal (Bio-Rad)), for each of the 4 dilution points, a total of 94 replicate reactions were distributed. In addition, the NTC reaction was repeated 8 times [28 (link)]. This dataset will be referred to as ‘94-replicates-4-dilutions set’. And 44 (4 × 11) amplification curves of the MYCN gene with a diluted concentration of 15, 150, 1500,15,000(11 replicated experiments for each group) were used for subsequent analysis.