Mirvana mirna mimic and inhibitor

The human CRC cell lines SW480 (ATCC CCL-228) and HT-29 (ATCC HTB-38) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). Authentication was done by the authors in all cases (LGC Standards, Wesel, Germany). Cell lines were maintained in RPMI-1640 (Invitrogen, Carlsbad, CA, USA) with 10% fetal bovine serum (FBS) and were grown at 37 °C in a 5% CO₂ atmosphere. Media were supplemented with penicillin G (100 U/mL) and streptomycin (0.1 mg/mL). For transfection experiments, CRC cells were seeded in 6-well plates and transfected with 10 µL of Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) and 20 nM of a miR-199b specific mirVana™ miRNA Mimic and Inhibitor (Ambion, Cambridge, UK).

The human CRC cell lines SW480 (ATCC CCL-228), WiDr (ATCC CCL-218), DLD-1 (ATCC CCL-221), HT-29 (ATCC HTB-38) and SW620 (ATCC CCL-227) were purchased from American Type Culture Collection (ATCC). Authentication was done by the authors in all cases (LGC Standards). Cell lines were maintained in RPMI-1640 (Invitrogen) with 10% fetal bovine serum and were grown at 37°C in a 5% CO2 atmosphere. Media were supplemented with penicillin G (100 U/ml), and streptomycin (0.1 mg/ml). Cells were treated with oxaliplatin (LOHP) (1 μM) (Sigma), 5-fluorouracil (5-FU) (1 μM) (Sigma) and FTY720 (10 μM) (Calbiochem) as previously reported [6 , 9 (link)]. For transfection experiments, CRC cells were seeded in 6-well plates and transfected with 10 μl of Lipofectamine 2000 (Life Technologies) and 2 μg of SET plasmidic vector or 20 nM of a miR-199b specific mirVana™ miRNA Mimic and Inhibitor (Ambion).