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Rneasy mini rna extraction kit

Manufactured by Thermo Fisher Scientific
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The RNeasy Mini Kit is a product by Thermo Fisher Scientific that is designed for the purification of total RNA from various biological samples. It utilizes a silica-membrane-based technology to efficiently capture and purify RNA molecules. The kit includes necessary buffers, reagents, and spin columns to facilitate the RNA extraction process.

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Lab products found in correlation

High capacity rna to cdna kit, by Thermo Fisher Scientific (3 mentions) Sybr green master mix, by Thermo Fisher Scientific (1 mentions) Taqman assay, by Thermo Fisher Scientific (1 mentions)

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RNeasy Mini Kit (282 citations) RNA extraction kit (258 citations) RNeasy kit (131 citations) RNA Mini Kit (57 citations) RNeasy extraction kit (10 citations) RNeasy Mini (5 citations)

3 protocols using rneasy mini rna extraction kit

1

Quantitative analysis of MAPT transcripts

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Cell pellets were collected in cold PBS by scraping, and RNA was extracted using the Qiagen RNeasy mini RNA extraction kit, then reverse transcribed using the high capacity RNA-to-cDNA kit (ThermoFisher Scientific). For qRTPCR of 3R and 4R MAPT transcripts, amplification was carried out using SybrGreen mastermix (ThermoFisher Scientific) with the following primers: MAPT 4R Forward 5’-CGGGAAGGTGCAGATAATTAA-3’, Reverse 5’-GCCACCTCCTGGTTTATGATG-3’; MAPT 3R Forward 5’-AGGCGGGAAGGTGCAAATA-3’, Reverse 5’-GCCACCTCCTGGTTTATGATG-3’.
Bowles K., Pugh D., Pedicone C., Oja L., Weitzman S., Liu Y., Chen J., Disney M, & Goate A. (2023). Development of MAPT S305 mutation models exhibiting elevated 4R tau expression, resulting in altered neuronal and astrocytic function. bioRxiv.
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2

Quantifying MAPT Isoform Expression

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Cell pellets were collected by washing and scraping into ice-cold PBS. RNA was extracted from cell pellets using the Qiagen RNeasy mini RNA extraction kit, and reverse transcribed using the high capacity RNA-to-cDNA kit (ThermoFisher Scientific). qRTPCR for specific MAPT exons and isoforms was carried out using SybrGreen mastermix with the following primers: MAPT 4R Forward 5’-CGGGAAGGTGCAGATAATTAA-3’, Reverse 5’-GCCACCTCCTGGTTTATGATG-3’; MAPT 3R Forward 5’-AGGCGGGAAGGTGCAAATA-3’, Reverse 5’-GCCACCTCCTGGTTTATGATG-3’; MAPT 0N Forward 5’-TTTGAACCAGGATGGCTGAG-3’, Reverse 5’-ATGCCTGCTTCTTCAGCTTT-3’; MAPT Exon 2 Forward 5’-TTTGAACCAGGATGGCTGAG-3’, Reverse 5’-CTGCAGGGGAGATTCTTTCA-3’. SF/RBP overexpression and knockdown was validated and quantified by qRTPCR using commercially available Taqman assays (ThermoFisher Scientific).
Bowles K.R., Pugh D.A., Oja L.M., Jadow B.M., Farrell K., Whitney K., Sharma A., Cherry J.D., Raj T., Pereira A.C., Crary J.F, & Goate A.M. (2021). Dysregulated coordination of MAPT exon 2 and exon 10 splicing underlies different tau pathologies in PSP and AD. Acta neuropathologica, 143(2), 225-243.
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3

Quantifying MAPT Isoform Expression

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Cell pellets were collected by washing and scraping into ice-cold PBS. RNA was extracted from cell pellets using the Qiagen RNeasy mini RNA extraction kit, and reverse transcribed using the high capacity RNA-to-cDNA kit (ThermoFisher Scientific). qRTPCR for specific MAPT exons and isoforms was carried out using SybrGreen mastermix with the following primers: MAPT 4R
Bowles K.R., Pugh D.A., Oja L., Jadow B.M., Farrell K., Whitney K., Sharma A., Cherry J.D., Raj T., Pereira A.C., Crary J.F., & Goate A. (2021). Dysregulated coordination ofMAPTexon 2 and exon 10 splicing underlies different tau pathologies in PSP and AD.
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