Phospho stat3 d3a7

Manufactured by Cell Signaling Technology

Sourced in United States

Phospho-STAT3 (D3A7) is a lab reagent that detects phosphorylated STAT3 protein. It is used to study the activation of the STAT3 signaling pathway.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using phospho stat3 d3a7

Hepatocyte Stress Signaling Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total liver lysates were analyzed by immunoblotting using antibodies against HBsAg (20-HR20, Fitzgerald), GADD153 (F-168, Santa Cruz), phospho-PERK (16F8, Cell Signaling), PERK (H-300, Santa Cruz), phospho-eIF2α (119A11, Cell Signaling), eIF2α (L57A5, Cell Signaling), β-actin (13E5, Cell Signaling), JNK2 (N-18, Santa Cruz), c-Jun (60A8, Cell Signaling), phospho-c-Jun (D47G9, Cell Signaling), phospho-SAPK/JNK (81E11, Cell Signaling), STAT3 (79D7, Cell Signaling), phospho-STAT3 (D3A7, Cell Signaling).

Churin Y., Roderfeld M., Stiefel J., Würger T., Schröder D., Matono T., Mollenkopf H.J., Montalbano R., Pompaiah M., Reifenberg K., Zahner D., Ocker M., Gerlich W., Glebe D, & Roeb E. (2014). Pathological Impact of Hepatitis B Virus Surface Proteins on the Liver Is Associated with the Host Genetic Background. PLoS ONE, 9(3), e90608.

+ Open protocol

+ Expand

Honokiol Signaling Pathway Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Honokiol (purity >98 %) was kindly provided by Dr. Jack L. Arbiser, Emory University, USA. It was dissolved in dimethyl sulfoxide (DMSO) and further diluted in sterile culture medium for in vitro experiments. The final concentrations of DMSO in cell cultures were all less than 0.05 %. The antibodies against Bax (B-9, mouse monoclonal antibody, sc-7480), Bcl-2 (100, mouse monoclonal antibody, sc-509), Erk (K-23, rabbit polyclonal antibody, sc-94), phospho-Erk (E-4, mouse monoclonal antibody, sc-7383) and STAT3 (F-2, mouse monoclonal antibody, sc-8019) were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). The antibodies against caspase 3 (5A1E, rabbit monoclonal antibody, #9664), Akt (5G3, mouse monoclonal antibody, #2966), phospho-Akt (587 F-11, mouse monoclonal antibody, #4051), JAK2 (D2E12, rabbit monoclonal antibody, #3230), phospho-JAK2 (D4A8, rabbit monoclonal antibody, #8082) and phospho-STAT3 (D3A7, rabbit monoclonal antibody, #9145) were obtained from Cell Signaling Technology (Beverly, MA, USA).

Huang J.S., Yao C.J., Chuang S.E., Yeh C.T., Lee L.M., Chen R.M., Chao W.J., Whang-Peng J, & Lai G.M. (2016). Honokiol inhibits sphere formation and xenograft growth of oral cancer side population cells accompanied with JAK/STAT signaling pathway suppression and apoptosis induction. BMC Cancer, 16, 245.

+ Open protocol

+ Expand

Immunomodulatory effects of compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Con A, chloroquine, and DHA were purchased from Sigma–Aldrich (St. Louis, MO, USA). Antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA), including the antibodies against p62 (Cat# 5114), LC3 (D11), phospho-STAT1 (58D6), STAT1 (D1K9Y), phospho-STAT3 (D3A7), STAT3 (D3Z2G), phospho-NF-κB p65 (93H1), NF-κB p65 (C22B4), and GAPDH (D16H11). Mouse monoclonal antibodies against CD3 (145-2C11), CD4 (RM4-5), IFN-γ (XMG1.2), and CD69 (H1.2F3) were purchased from BD Pharmingen (San Jose, CA, USA). Anti-NK1.1 (PK136), anti-CD16/CD32 (2.4G2), goat anti-rabbit IgG, and 7-aminoactinomycin D (7-AAD) were from MultiSciences (Hangzhou, China). Also, 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE) was purchased from Invitrogen (San Diego, CA, USA).

Li Y., Tang Y., Wang S., Zhou J., Zhou J., Lu X., Bai X., Wang X.Y., Chen Z, & Zuo D. (2016). Endogenous n-3 Polyunsaturated Fatty Acids Attenuate T Cell-Mediated Hepatitis via Autophagy Activation. Frontiers in Immunology, 7, 350.

+ Open protocol

+ Expand

Protein Analysis of Liver Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sample preparation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting was performed as described before [48 (link)]. Total liver lysates were analyzed by using antibodies against HBsAg (20-HR20, Fitzgerald, Acton, Ma, USA), phospho-PERK (16F8, Cell Signaling, Beverly, MA, USA), phospho-eIF2α (119A11, Cell Signaling), β-actin (13E5, Cell Signaling), c-Jun (60A8, Cell Signaling), phospho-c-Jun (D47G9, Cell Signaling), phospho-SAPK/JNK (81E11, Cell Signaling), STAT3 (79D7, Cell Signaling), phospho-STAT3 (D3A7, Cell Signaling).

Zahner D., Glimm H., Matono T., Churin Y., Herebian D., Mayatepek E., Köhler K., Gattenlöhner S., Stinn A., Tschuschner A., Roderfeld M, & Roeb E. (2017). Hepatitis B virus surface proteins accelerate cholestatic injury and tumor progression in Abcb4-knockout mice. Oncotarget, 8(32), 52560-52570.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!