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Interleukin 6 (il 6)

Manufactured by Cusabio
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Sourced in China, United States

IL-6 is a laboratory reagent used in research applications. It is a cytokine that plays a role in immune and inflammatory responses. The product's core function is to facilitate the study of IL-6 and its related biological processes.

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Lab products found in correlation

Il 1β, by Cusabio (17 mentions) Tnf α, by Cusabio (15 mentions) Il 10, by Cusabio (7 mentions) Caspase 3, by Cusabio (3 mentions) Bcl2 associated x (bax), by Cusabio (3 mentions) C myc, by Cusabio (3 mentions) Bcl 2, by Cusabio (3 mentions) Ifn γ, by Cusabio (3 mentions) Tgf β, by Cusabio (3 mentions) Il 10, by Thermo Fisher Scientific (3 mentions) Mcp 1, by Cusabio (2 mentions) K ras, by Cusabio (2 mentions) Microplate reader, by Thermo Fisher Scientific (2 mentions) β actin, by Santa Cruz Biotechnology (2 mentions) Endothelin 1, by R&D Systems (1 mentions) Hs crp, by Cusabio (1 mentions) 8 iso pgf2α, by Cayman Chemical (1 mentions) Tnf α, by R&D Systems (1 mentions) Cx3cl1, by Cloud-Clone (1 mentions) Cd200, by Cusabio (1 mentions)

Close spelling variants of this product

Interleukin-6 (IL-6), (2 citations) Mouse Interleukin 6 [IL-6] ELISA Kit (2 citations)

40 protocols using interleukin 6 (il 6)

1

Cytokine Profile Quantification

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Concentration of TNF-α, IL-6, IL-10, and IL-RA in cell supernatant were determined using commercial kits followed with instruction: TNF-α (Cusabio, Wuhan, China; cat: CSB-E04740h), IL-6 (Cusabio, cat: CSB-E04638h), IL-10 (Cusabio, cat: CSB-E04593h), and IL-RA (Cusabio, cat: CSB-E04629h).
Gong B., Zheng Y., Li J., Lei H., Liu K., Tang J, & Peng Y. (2022). Luteolin activates M2 macrophages and suppresses M1 macrophages by upregulation of hsa_circ_0001326 in THP-1 derived macrophages. Bioengineered, 13(3), 5079-5090.
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2

Cardiovascular biomarkers in plasma

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Blood samples were obtained by abdominal aorta punction. Blood was collected in heparinized syringes and plasma was separated by centrifugation and stored at −80 °C until assayed.
Plasma creatinine, phosphate, magnesium, glucose, total cholesterol, high-density lipoprotein (HDL)-cholesterol and urine phosphate and magnesium were measured by spectrophotometry (Bio-Systems, Barcelona, Spain). No-HDL cholesterol values were obtained subtracting the values of total cholesterol the values of HDL.
ELISA tests were used to determine plasma levels of Endothelin-1 (R&D Systems, Minneapolis, MN, USA), Fibroblast Growth Factor 23 (FGF23) (Kainos Laboratories, Tokyo, Japan) and IL-6 (Cusabio, Wuhan, China). Plasma nitric oxide (NO) was quantified by the commercial kit QuantiChrom (BioAssay Systems, Hayward, CA, USA) which is designed to accurately measure NO production following reduction of nitrate to nitrite using the Griess method and reading its absorbance at 540 nm.
López-Baltanás R., Rodríguez-Ortiz M.E., Díaz-Tocados J.M., Martinez-Moreno J.M., Membrives C., Rodelo-Haad C., Pendón Ruiz de Mier M.V., Rodríguez M., Canalejo A., Almadén Y, & Muñoz-Castañeda J.R. (2023). Dietary Mg Supplementation Decreases Oxidative Stress, Inflammation, and Vascular Dysfunction in an Experimental Model of Metabolic Syndrome with Renal Failure. Antioxidants, 12(2), 283.
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3

Serum Biomarker Profiling Protocol

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Serum levels of TRAIL (Senxiong Biotech, Shanghai, China), interleukin (IL)-1 (Beijing 4A Biotech Co., Ltd), IL-6 (CUSABIO, Wuhan, China), TNF-α (CUSABIO), monocyte chemotactic protein-1 (MCP-1) (CUSABIO) and hypersensitive C-reactive protein (hs-CRP) (CUSABIO) were measured by using commercially available enzyme-linked immunosorbent assays, according to the manufacturers’ instructions.
Chang W.W., Liang W., Yao X.M., Zhang L., Zhu L.J., Yan C., Jin Y.L, & Yao Y.S. (2018). Tumour necrosis factor-related apoptosis-inducing ligand expression in patients with diabetic nephropathy. Journal of the Renin-Angiotensin-Aldosterone System: JRAAS, 19(3), 1470320318785744.
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4

Spinal Cord Ischemia-Induced Oxidative Damage

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Oxidative damage induced by spinal cord ischemia was assessed by measuring MDA and 8-iso-PGF2α, which are generated by the peroxidation of polyunsaturated fatty acid and arachidonic acid in the membrane, respectively [55 (link),56 (link)]. The inflammatory response induced by spinal cord ischemia was evaluated by measuring pro-inflammatory cytokines, such as IL-1β, IL-6, and TNF-α. Briefly, animals (n = 5 per group) were anesthetized using 5% isoflurane, and the lumbar spinal cords (L6–L7) were obtained. MDA (Cayman Chemical Company, Ann Arbor, MI, USA), 8-iso-PGF2α (Cayman Chemical Company), IL-1β (Cusabio, Hubei, China), IL-6 (Cusabio), and TNF-α (R&D Systems Inc., Minneapolis, MN, USA) ELISA assay kits were used to measure the oxidative indicators and pro-inflammatory cytokines as per the manufacturer instructions.
Jung H.Y., Kwon H.J., Kim W., Hahn K.R., Moon S.M., Yoon Y.S., Kim D.W, & Hwang I.K. (2020). Phosphoglycerate Mutase 1 Prevents Neuronal Death from Ischemic Damage by Reducing Neuroinflammation in the Rabbit Spinal Cord. International Journal of Molecular Sciences, 21(19), 7425.
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5

Serum Cytokine Profiles in Surgical Patients

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Blood was collected prior to and at 4, 9 days after surgery and centrifuged at 4,000 x g for 10 min at 4˚C to prepare the serum. Concentrations of TNF-α (cat. no. CSB-E11987r; Cusabio Technology), IL-6 (cat. no. CSB-E04640r; Cusabio Technology), IL-1β (cat. no. CSB-E08055r; Cusabio Technology) and IL-10 (cat. no. CSB-E04595r; Cusabio Technology) in serum were determined using ELISA kits following the manufacturer's instructions.
Suo L, & Wang M. (2021). Dexmedetomidine facilitates the expression of nNOS in the hippocampus to alleviate surgery-induced neuroinflammation and cognitive dysfunction in aged rats. Experimental and Therapeutic Medicine, 22(3), 1038.
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6

Cytokine and Apoptosis Protein Assay Protocol

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2-ME (C3H8O2; CAS# 109-84-4; 99.5% purity), is a product of BDH Laboratory Supplies, Poole, BH15 1TD, England. Rats TNF-α (CSB-E11987r), IL-1β (CSB-E08055r), IL-6 (CSB-E04640r), IL-10 (CSB-E04595r), caspase-3 (CSB-E08857r), p53 (CSB-E08336r), Bax (CSB-EL002573RA), Bcl-2 (CSB-E08854r), c-Myc (CSB-E09260h), and K-Ras (CSB-EL012493h) enzyme-linked immunosorbent assay (ELISA) kits are products of Cusabio Technology Llc, Houston, TX, USA. All other used chemicals and reagents were of analytical grade and were products of Sigma Chemical Co., Saint Louis, MO, USA or BDH Chemical Ltd, Poole, England.
Somade O.T., Ajayi B.O., Olunaike O.E, & Jimoh L.A. (2020). Hepatic oxidative stress, up-regulation of pro-inflammatory cytokines, apoptotic and oncogenic markers following 2-methoxyethanol administrations in rats. Biochemistry and Biophysics Reports, 24, 100806.
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7

Hippocampal Cytokine Profiling in Stress

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ELISA method was used to measure the content of IL-1β, IL-6 (both obtained from CUSABIO, Wuhan, China), and IL-10 (BOOST Biotech, Wuhan, China) in rat hippocampus exposed to the SPS paradigm according to the manufacturer’s instructions of kits. Briefly, the hippocampus was separated and homogenized in PBS containing protease inhibitors. The tissue homogenate was centrifuged at 13,000×g for 20 min at 4 °C, and the supernatant was collected and immediately stored at − 80 °C until further processing.
Nie P.Y., Tong L., Li M.D., Fu C.H., Peng J.B, & Ji L.L. (2021). miR-142 downregulation alleviates rat PTSD-like behaviors, reduces the level of inflammatory cytokine expression and apoptosis in hippocampus, and upregulates the expression of fragile X mental retardation protein. Journal of Neuroinflammation, 18, 17.
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8

Quantification of Neuroinflammatory Markers in Rat Brain

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The protein levels of CX3CL1 (Cloud-Clone Corp., Katy, TX, USA), CX3CR1, CD200, CD200R, IL-6, and IL-4 (all from Cusabio, Houston, TX, USA) in the frontal cortices and hippocampi of the male rats were measured using commercially available ELISA kits. The procedures were performed in accordance with the manufacturer’s instructions and the minimum detectable doses were: CX3CL1: 0.055 ng/mL, CX3CR1: 5.8 pg/mL, CD200: 11.75 pg/mL, CD200R: 4.67 pg/mL, IL-6: 0.78 pg/mL, IL-4: 3.9 pg/mL. Intra- and inter-assay precision values were: CX3CL1: <10%, <12%, CX3CR1, CD200, CD200R, IL-6, IL-4: <8%, and <10%, respectively.
Chamera K., Szuster-Głuszczak M., Trojan E, & Basta-Kaim A. (2020). Maternal Immune Activation Sensitizes Male Offspring Rats to Lipopolysaccharide-Induced Microglial Deficits Involving the Dysfunction of CD200–CD200R and CX3CL1–CX3CR1 Systems. Cells, 9(7), 1676.
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9

Quantifying IL-6 and IL-10 in THP-1 cells

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After the polarisation of THP-1 cells, the supernatant of each group of cells was collected. IL-6 (CUSABIO, Wuhan, China) and IL-10 (eBioscience, Austria) were detected according to the procedure of the enzyme-linked immunosorbent assay (ELISA) kit. Absorbance was recorded at a wavelength of 450 nm using a microplate reader (Thermo Scientific, USA). The expression levels of IL-6 and IL-10 were calculated according to the standard curve.
Liu Q., Liu Y., Guan X., Wu J., He Z., Kang J., Tao Z, & Deng Y. (2019). Effect of M2 Macrophages on Injury and Apoptosis of Renal Tubular Epithelial Cells Induced by Calcium Oxalate Crystals. Kidney & blood pressure research, 44(4).
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10

Hepatoprotective Effects of EGME

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EGME (C3H8O2; CAS# 109-84-4; 99.5% purity), is a product of BDH Laboratory Supplies, Poole, BH15 1TD, England. Rats IL-10, IL-6, TNF-α, IL-1β, caspase-3, p53, Bax, Bcl-2, c-Myc, and K-Ras enzyme-linked immunosorbent assay (ELISA) kits are manufactured by Cusabio Technology Llc, Houston, TX, USA. The rest of the reagents and chemicals used were obtained from a recognized chemical manufacturing company and were of standard and analytical grade.
Somade O.T., Ajayi B.O., Adeyi O.E., Adeshina A.A., James A.S, & Ayodele P.F. (2020). Ethylene glycol monomethyl ether-induced testicular oxidative stress and time-dependent up-regulation of apoptotic, pro-inflammatory, and oncogenic markers in rats. Metabolism Open, 7, 100051.
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