Uplc xevo tq s

Manufactured by Waters Corporation

Sourced in United States

The UPLC-Xevo TQ-S is a high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) system designed for analytical applications. It combines a ultra-high performance liquid chromatography (UPLC) system with a triple quadrupole mass spectrometer to provide rapid, sensitive, and selective analysis of complex samples.

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Lab products found in correlation

Sas 9, by SAS Institute (2 mentions) Phoenix winnonlin software, by Certara (1 mentions)

Close spelling variants of this product

Xevo TQ-S UPLC-MS/MS system ACQUITY UPLC I-Class/Xevo TQ-S ACQUITY UPLC I‐Class/Xevo TQ‐S micro System ACQUITY UPLC-Xevo TQ-S Acquity I Class UPLC/Xevo TQ-S Micro Mass Spectrometer Xevo TQ-S mass spectrometry (UPLC–MS/MS, Waters ACQUITY UPLC-Xevo TQ-S Acquity UPLC-Xevo TQ-S system ACQUITY UPLC I-Class-Xevo TQ-S Micro Xevo TQ-S

7 protocols using uplc xevo tq s

Pharmacokinetics and Immunogenicity Analysis

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All statistical analyses were done with the SAS 9.4 (SAS Institute) in the Statistical Analysis Department, Jiaxing Taimei Medical Technology. The pharmacokinetic parameters and ADA indexes were tested and analysed by Joinn Laboratories (China) Co., Ltd. The pharmacokinetic parameters were estimated using the Phoenix WinNonlin software (Certara).
All the pharmacokinetic samples in the single and multiple dosing tests were collected, centrifuged and packed by the research centre and transported to the testing unit in the frozen state on dry ice (≤−70℃). They were tested and analysed for the plasma concentration using the validated LC‐MS/MS (Waters, UPLC‐Xevo TQ‐S) analysis method, and the quantitative lower limit is 0.22 ng/mL for the SAD and 0.3 ng/mL for MAD. The non‐compartment model (NCA) was used to calculate the pharmacokinetic parameters, and the main parameters obtained were C_max, T_max, AUC, t_1/2, CL and V_z.
All the ADA samples are transported to the testing unit in the cold chain under dry ice (≤−70℃) and were screened and confirmed with the indirect ELISA analysis method, and the positive samples were tested for the antibody titre.

Wang X., Liu L., Qi L., Lei C., Li P., Wang Y., Liu C., Bai H., Han C., Sun Y, & Liu J. (2021). A first‐in‐human, randomized, double‐blind, single‐ and multiple‐dose, phase I study of recombinant human thymosin β4 in healthy Chinese volunteers. Journal of Cellular and Molecular Medicine, 25(17), 8222-8228.

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Phytohormone Extraction and Analysis

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To measure endogenous phytohormone contents, ∼200 mg of all aerial tissues, except the developed leaf blade, was collected before and after submergence. Each sample was collected into a 2.0 mL Master-Tube hard (Qiagen, Turnberry Lane, Valencia, CA, USA), frozen and crushed with four iron beads in liquid nitrogen. The concentration of endogenous GA was measured using UPLC-MS/MS (UPLC-Xevo TQ-S; Waters, Maple Street, Milford, MA, USA) at the Institute of Plant Productivity Systems Research Group RIKEN Center for Sustainable Resource Science. Each compound was measured as described previously (Kojima et al. 2009 (link)).

Ayano M., Kani T., Kojima M., Sakakibara H., Kitaoka T., Kuroha T., Angeles-Shim R.B., Kitano H., Nagai K, & Ashikari M. (2014). Gibberellin biosynthesis and signal transduction is essential for internode elongation in deepwater rice. Plant, Cell & Environment, 37(10), 2313-2324.

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Quantitative Mouse Blood Metabolomics

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The mouse blood samples were quantitatively analyzed with high sensitivity by a tandem quadrupole mass spectrometer (UPLC XEVO TQ-S, Waters, USA). Chromatographic separation was executed on an UPLC RP Column (2.1 mm × 150 mm, 1.8 μm). The analytical column temperature was sustained at 40°C, and the flow rate was 0.6 mL/min. The mobile phase comprised solution A (0.1% formic acid in water) and solution B (acetonitrile and water, 95:5). The gradient elution was optimized as follows: 0 to 0.5 min, 96% to 4% B; 0.5 to 2.5 min, 90% to 10% B; 2.5 to 5 min, 72% to 28% B; 5 to 7 min, 5% to 95% B; and 7 to 9 min, 96% to 4% B. Multiple reaction monitoring analyses were carried out adopting a Xevo TQ-S mass spectrometer. All experiments were executed in positive electrospray ionization mode. The capillary voltage and ion source temperature were kept invariant and set to 2.0 kV and 150°C. The cone gas flow rate, desolvation temperature, and desolvation gas flow were 150 L/h, 600°C, and 1000 bar, respectively. The system was commanded by Masslynx software.

Wang W., Liu T, & Zhang Y. (2023). An integrated targeted metabolomics and network pharmacology approach to exploring the mechanism of ellagic acid against sleep deprivation-induced memory impairment and anxiety. Digital Health, 9, 20552076231169846.

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Quantification of Serum Vitamins A, D, and E

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The samples were analyzed at the Neonatal Screening Center, Chongqing Health Center for Women and Children. The Vitamin A, D and E concentrations in serum were measured by high-performance liquid chromatography tandem-mass spectrometry (HPLCMS/MS) using a Waters UPLC Xevo TQS (Waters Corporation, Milford, MA, USA) described by Hao Liu et al. (16 (link)). Briefly, QCs, standards, and plasma samples were added into the corresponding wells of 96-wells plates. Protein was precipitated by adding isotopic IS in a 35:1 (v/v) mixture of methanol: isopropanol, then hexane was added to each well for extraction. The Vitamin A, D and E in serum was separated by HPLC on a Shimadzu Waters BEH C18μm 1.7 2.1*50mm column and quantitated by MS.

Yang G., wang N., Liu H., Si L, & Zhao Y. (2023). The association between umbilical cord blood fat-soluble vitamin concentrations and infant birth weight. Frontiers in Endocrinology, 14, 1048615.

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Pharmacokinetics of GS-441514 in Rats

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SD rats (male, four animals per group) weighing 180-220 g were injected with GS-441514 intravenously (iv) and intragastricly (ig) at a dose of 30mg/kg. After administration, 0.3 mL of the orbital blood was taken at 0.083, 0.16 0.25, 0.5, 2, 3, 4, 8, 24 and 48 h for the iv group, and 0.25, 0.5, 1, 2, 3.0, 4, 6, 8, 24 and 48 h for the ig group, respectively. Samples were centrifuged under 4000 rpm/min for10 min at 4℃. The supernatants (plasma) were collected and stored at -20℃ for future analysis. For plasma drug concentration analysis, an aliquot of 50 ul each plasma sample was treated with 100ul of 90% methanol and 600 ul of 50% acetonitrile mixture. The samples were centrifuged under 1200 rpm for 10min and filtered through 0.2 μm membrane filters. The drug concentration in each sample was tested by HPLC/MS. Analytes were separated on a InertSustain AQ-C18HP column (3.0 mm× 50 mm, 3.0µm, GL) using Waters UPLC/XEVO TQ-S. The pharmacokinetic parameters were calculated using DAS (Drug and Statistics) 3.0 software. The time-concentration curve was plotted using GraphPad Prism 6 software.

Li Y., Cao L., Li G., Cong F., Li Y., Sun J., Luo Y., Chen G., Li G., Wang P., Xing F., Ji Y., Zhao J., Zhang Y., Guo D, & Zhang X. (2022). Remdesivir Metabolite GS-441524 Effectively Inhibits SARS-CoV-2 Infection in Mouse Models. Journal of medicinal chemistry, 65(4).

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Cytokinin Flux Measurement in Rice

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Five hills with the normal number of panicles were collected per replicate at 14 days after heading in 2011, and flag leaves were collected from the main tillers. To obtain exudates, shoots were cut 10 cm above the soil surface, and a polyethylene bag containing cotton wool was attached to each cut end with tape. Bags were detached 3 h later, sealed and weighed. The weight of the exudate was calculated by subtracting the weight of the bag and the cotton wool. Exudates were collected by squeezing the cotton wool using syringe and immediately stored at −80°C. Cytokinins were extracted using an automatic liquid handling system (MICROLAB STAR, Hamilton, NV, USA) for solid phase extraction and quantified by ultra-performance liquid chromatography (UPLC) coupled with a tandem quadrupole mass spectrometer (qMS/MS) equipped with an electrospray interface (ESI; UPLC-ESI-qMS/MS, UPLC-Xevo TQ-S, Waters, MA, USA) as described previously38 (link). Cytokinin flux was calculated from the exudation rate and cytokinin concentration.

Arai-Sanoh Y., Takai T., Yoshinaga S., Nakano H., Kojima M., Sakakibara H., Kondo M, & Uga Y. (2014). Deep rooting conferred by DEEPER ROOTING 1 enhances rice yield in paddy fields. Scientific Reports, 4, 5563.

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Hair Sample Extraction and Analysis

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Pulverizing of hair samples and first step extraction were performed by Lysera Bead Mill Homogenizer, Biotage (Uppsala, Sweden). A QUINTIX65-1S electronical scale was used for weighing the hair samples purchased from Sartorius (Göttingen, Germany). MAX µElution Oasis HLB solid phase extraction (SPE) and positive pressure manifold used for the second step of extraction/clean-up were both from Waters (Manchester, UK). Apricot designs MT Evaporex EVX-192 Perkin Elmer (Waltham, USA) was used to dry the samples using nitrogen gas before and after the SPE step. Sample measurements were performed by reverse-phase liquid chromatography (UPLC) using UPLC-Xevo-TQS and an analytical column, Acquity UPLC HSS T3 (2.1 × 100 mm, 1.8 µm), both from Waters.

Mirzaian M., van Zundert S.K.M., Schilleman W.F., Mohseni M., Kuckuck S., van Rossum E.F.C., van Schaik R.H.N, & van den Berg S.A.A. (2024). Determination of cortisone and cortisol in human scalp hair using an improved LC-MS/MS-based method. Clinical chemistry and laboratory medicine, 62(1).

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