Terbuthylazine

Manufactured by Merck Group

Sourced in Germany

Terbuthylazine is a chemical compound used as a laboratory standard for analytical purposes. It is a white crystalline solid with the molecular formula C₉H₁₆ClN₅. Terbuthylazine is commonly used as a reference material in the analysis and identification of similar compounds.

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Lab products found in correlation

4 protocols using terbuthylazine

Pesticide Concentrations in Signal Crayfish Exposure

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Metazachlor (MTZ), chemical purity 99.7%; terbuthylazine (TER), chemical purity 99.4%; and thiacloprid (TCL), chemical purity 99.9%, were purchased from Sigma-Aldrich Corporation (USA). Chemicals were dissolved in dechlorinated tap water to obtain 20 μg/L, 4 μg/L, and 6 μg/L for MTZ, TER, and TCL, respectively. Actual concentrations of chemicals in water during the experiments were within 96% of the nominal concentrations (Table 2). The analyses of pesticides in water were performed by the State Research Institute in Prague using methods described by Anastassiades et al. (2003 (link)) and Anastassiades et al. (2007 ).Table 2

Concentrations of Metazachlor (MTZ), terbuthylazine (TER), and thiacloprid (TCL) in exposure and control groups of signal crayfish Pacifastacus leniusculus

Group	Tank (n)	Nominal concentration (µg/L)	Concentration (µg/L)Mean ± SD	t	p-value
MTZ	6	20	19.3±1.5	-1.2	0.28
Control	6	˗	< 0.010	˗	˗
TER	6	4	3.9 ± 0.1	− 1.93	0.11
Control	6	˗	< 0.010	˗	˗
TCL	6	6	5.7 ± 0.3	− 2.18	0.08
Control	6	˗	< 0.010	˗	˗

t, t-score. p < 0.05. The limit of detection for the concentrations was 0.010 µg/L

Malinovska V., Kuklina I., Lozek F., Velisek J, & Kozak P. (2023). Responses of signal crayfish Pacifastacus leniusculus to single short-term pulse exposure of pesticides at environmentally relevant concentrations. Environmental Science and Pollution Research International, 30(18), 51740-51748.

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Herbicide Degradation Experiment Protocol

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Terbuthylazine (TBA), atrazine (ATZ) (both Pestanal, purity 99.1%) and urea (≥ 95%) were purchased from Sigma-Aldrich (Seelze, Germany), (NH₄)₂SO₄ from Panreac (Barcelona, Spain) and NaNO₃ from Merck (Darmstadt, Germany). The formulated herbicide Terbutilazina-Sapec (concentrate suspension of 0.5 kg active substance L^-1; recommended field dose (RD) for weed control in corn plantations of 1.5 L ha^-1) was purchased from Sapec—Agro (Setúbal, Portugal).

Silva V.P., Moreira-Santos M., Mateus C., Teixeira T., Ribeiro R, & Viegas C.A. (2015). Evaluation of Arthrobacter aurescens Strain TC1 as Bioaugmentation Bacterium in Soils Contaminated with the Herbicidal Substance Terbuthylazine. PLoS ONE, 10(12), e0144978.

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Pesticide Degradation Using Wood Shavings

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Terbuthylazine, tebuconazole, and alachlor (PESTANAL^® product line) were obtained from Sigma-Aldrich (Taufkirchen, Germany) at a purity higher than 98%. Pesticide stock solutions were prepared in analytical-grade methanol at a concentration of 1000 mg L⁻¹ and stored in the dark at 4 °C.
Wood shavings were used as a bacterial carrier and a source of organic carbon. The wood shavings were obtained from poplar trees growing in the territory of the Slovak Republic. After cutting the trees to shavings, these were sieved at the 1.0–1.5 cm fraction.
Argon gas, at the purity of 99.996%, was obtained from Linde Gas (Prague, Czech Republic).

Pániková K., Bílková Z, & Malá J. (2023). The Behavior of Terbuthylazine, Tebuconazole, and Alachlor during Denitrification Process. Journal of Xenobiotics, 13(4), 560-571.

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Pesticide Standards Preparation and Characterization

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Atrazine, Atrazine-desethyl (DEA), Atrazine-desisopropyl (DIA), bromacil, carbendazim, chlorpyrifos, diuron, imazalil, linuron, metolachlor, prometryn, simazine, terbumeton, terbumeton-desethyl (TED) , terbuthylazine, terbuthylazine-desethyl (TD), terbutryn, thiabendazole, 3,4-dichloroaniline (3,4-DCA), 3,5,6-trichloro-2-pyridinol (TCP) were purchased from Sigma-Aldrich (Madrid, Spain), with a purity higher than 97%. The list of pesticides and TPs available for this study, as well as some physicochemical properties, are listed in Supplementary Material (Table SM1).
HPLC-grade methanol (MeOH), acetone (pesticide residue analysis quality) and formic acid (98-100%, LC-MS grade), were supplied by Scharlau S.L (Barcelona, Spain).
HPLC-grade water (resistivity of 18 MΩ cm) was obtained by purifying demineralised water (Millipore Ltd., Bedford, MA, USA).
Standard stock solutions (500 mg L -1 ) of each compound (except carbendazim) were prepared by dissolving 25 mg (±0.01 mg) of each solid compound in 50 mL of acetone considering the purity of each solid standard. Standard stock solution of carbendazim was prepared in MeOH. Individual stock solutions and working standard solutions were stored at -20 and 4℃ in the dark, respectively.

Fonseca E., Renau-Pruñonosa A., Ibáñez M., Gracia-Lor E., Estrela T., Jiménez S., Pérez-Martín M.Á., González F., Hernández F, & Morell I. (2019). Investigation of pesticides and their transformation products in the Júcar River Hydrographical Basin (Spain) by wide-scope high-resolution mass spectrometry screening. Environmental research, 177.

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