A 500-denier 91:9% nylon:spandex plain-weave textile with a weight of 100 g/m2 was supplied by Stedfast Inc. (Granby, QC, Canada). Zinc chloride, anhydrous (ZnCl2, 97%, Alphachem, Mississauga, ON, Canada), silver nitrate (AgNO3, 99.5%, Thermo Fisher Scientific, Waltham, MA, USA), tea tree oil (Sigma-Aldrich, Burlington, MA, USA), trimethoxysilylpropyl octadecyldimethyl ammonium chloride (HM4072, 72%, Gelest, Morrisville, PA, USA), and (trihydroxysilyl) propyldimethyl octadecyl ammonium chloride (HM4005, 84%, Gelest, Morrisville, PA, USA) were purchased and used as antimicrobial agents.
Tea tree oil
Manufactured by Merck Group
Sourced in United States
Tea tree oil is a natural essential oil derived from the leaves of the Melaleuca alternifolia plant. It is a clear, pale yellow liquid with a distinctive, camphor-like aroma. Tea tree oil is commonly used in various laboratory and research applications due to its antimicrobial and antifungal properties.
Automatically generated - may contain errors
Lab products found in correlation
Silver nitrate agno3, by Thermo Fisher Scientific (1 mentions)
Peppermint oil, by Merck Group (1 mentions)
Sandalwood oil, by Merck Group (1 mentions)
Anise oil, by Merck Group (1 mentions)
Oregano oil, by Merck Group (1 mentions)
Eucalyptus oil, by Merck Group (1 mentions)
Thyme oil, by Merck Group (1 mentions)
Garlic oil, by Merck Group (1 mentions)
Oleic acid, by Gattefosse (1 mentions)
Transcutol, by Gattefosse (1 mentions)
Rhamnolipid, by Merck Group (1 mentions)
Poloxamer 407, by BASF (1 mentions)
Congo red, by Merck Group (1 mentions)
Calcofluor white, by Merck Group (1 mentions)
Sulforhodamine b, by Merck Group (1 mentions)
Trichloroacetic acid, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Lonza (1 mentions)
Dulbecco s modified eagle medium, by Lonza (1 mentions)
Phosphate buffered saline (pbs), by Lonza (1 mentions)
5 protocols using tea tree oil
1
Antimicrobial Textile Functionalization
2
Topical Antimicrobial Formulation Development
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Acyclovir was purchased from Acros Organics, Morris Plains, NJ, USA. Tea tree oil, garlic oil, peppermint oil, sandalwood oil, anise oil, oregano oil, chamomile oil, lemon palm oil, thyme oil, and eucalyptus oil were procured from Sigma Aldrich, St. Louis, MO, USA. Oleic acid, PEG 200®, PEG 400®, Labrasol® Propylene Glycol® and Transcutol®, Tween80®, span80®, tween60®, span60®, tween20®, span20®, hydroxyl propyl cellulose, and silicone were purchased from Gattefosse (Saint Priest Cedex, France). The chemicals used in the study consisted of analytical grade.
3
Tanshinone II A-based Anti-inflammatory Formulation
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Tanshinone II A (purity 98 %) was purchased from Baoji Guokang Bio-Technology Co., Ltd (China). Rhamnolipids and tea tree oil were obtained from Sigma-Aldrich (USA). Poloxamer 407 (P407) was purchased from BASF Co. (Germany). Propylene glycol (PG) and ethanol were from ADWIC, El-Nasr Pharmaceutical Chemicals Co. (Egypt). Lipopolysaccharide (LPS) was extracted by the Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Egypt. Thiobarbituric acid (TBA), protease inhibitor cocktail and other reagents used in the in vivo study were obtained from Sigma-Aldrich (USA). All other chemicals and organic solvents were of analytical grade.
4
Yeast Growth Media Optimization
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Standard procedures were used for yeast extract/peptone/dextrose media (YPD) and synthetic media (SD). The chemicals were added to plates with the final concentrations of 0.0075% SDS, 10ug/ml Calcofluor White (Sigma 18909), 10ug/ml Congo Red, (Sigma C-6767) or 0.15% Tea Tree Oil (Sigma SMB00386). 0.5% Tween 40 was added to the Tea Tree Oil and control plates to assure solubility of the Tea Tree Oil. In Fig 1B and 1D , supplements were spread on top of YPD + SDS plates at 1X final concentration from 100X stocks supplied at 6g/L adenine, 2g/L histidine, 12g/L leucine, 8g/L tryptophan or 2g/L uracil. The plates in Fig 2B contain additional 1X tryptophan (80mg/L), tyrosine (30mg/L) or phenylalanine (50mg/L) supplied to the media. YPD plates were incubated for two days and SD plates for three days at 30°C unless otherwise indicated. Images are representative of three independent experiments.
5
Evaluating Clindamycin and Propolis Wound Healing
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Clindamycin hydrochloride was kindly gifted by European Egyptian pharmaceutical industry company, Alexandria, Egypt, and lyophilized red propolis extract was purchased from VACSERA, Giza, EGYPT, tea tree oil was purchased from Sigma – Aldrich, Mumbai, India. All other reagents and solvents were of HPLC analytical grade obtained from Fisher Scientific Company, USA.
Dulbecco's Modified Eagle Medium, penicillin–streptomycin (100×), fetal bovine serum, and phosphate-buffered saline were purchased from Lonza Group Ltd., Basel, Switzerland. Sulforhodamine-B and trisaminomethane base were purchased from Sigma-Aldrich, Louis, MO, USA. Trichloroacetic acid was purchased from Merck, Darmstadt, Germany.
Sprague–Dawley rats without skin damage or diseases were obtained from Misr university for science and technology animal center (Giza, Egypt). The ethical committee approved all animal and cell lines studies of Misr university for science and technology (Approval No: PH 7).
Dulbecco's Modified Eagle Medium, penicillin–streptomycin (100×), fetal bovine serum, and phosphate-buffered saline were purchased from Lonza Group Ltd., Basel, Switzerland. Sulforhodamine-B and trisaminomethane base were purchased from Sigma-Aldrich, Louis, MO, USA. Trichloroacetic acid was purchased from Merck, Darmstadt, Germany.
Sprague–Dawley rats without skin damage or diseases were obtained from Misr university for science and technology animal center (Giza, Egypt). The ethical committee approved all animal and cell lines studies of Misr university for science and technology (Approval No: PH 7).
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