Human tgf β1 elisa kit

To measure TGF-β1 released by organoids or corresponding fibroblasts, cells were seeded into 6-well plates and stimulated with arecoline at the indicated concentrations (10 μg/mL) or not for 48 h. Then, the supernatant was harvested and the concentrations of TGF-β1 were measured using a human TGF-β1 ELISA kit (EHC107b.96, Neo Bioscience Technology, China) according to the manufacturer’s instructions. To confirm the THBS1 secretion of fibroblast with THBS1 knockdown or overexpression, the supernatant was harvested and the concentrations of THBS1 were measured using a Human THBS1/TSP1 ELISA Kit (EK0899, Raybiotech, USA).

Human IL-10 and human TGF-β1 ELISAs were performed using the human IL-10 ELISA kit and human TGF-β1 ELISA kit (NeoBioscience, Shenzhen, China) according to the manufacturer’s protocols. Absorbency was measured using a standard ELISA microplate reader (Thermo Fisher Scientific, Vantaa, Finland). Unused reagents after the experiment are completed, stored back in a refrigerator to restore temperature.