Anti flag tag

For immunoblotting, cells were lysed using RIPA buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS) (Thermo Fisher Scientific) supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor followed by centrifugation at 15,000×g for 10 min at 4 °C. Protein concentration was measured using the DC Protein Assay Kit (Bio-Rad) and equal amount of proteins were analyzed by SDS-PAGE and western blot analysis using standard procedure.
For co-immunoprecipitation experiments, cells were lysed using NP40 Lysis buffer (Boston BioProducts). Equal amounts of cleared cell lysates were adjusted to the same volume and incubated with anti-Flag tag (Sigma Aldrich) or anti-Myc tag (Thermo Fisher Scientific) magnetic beads for overnight incubation at 4 °C with gentle agitation. Beads were washed four times before elution with SDS sample buffer for immunoblot analysis.
Antibodies used in this study are anti-Flag tag (Cat# F1804, Sigma Aldrich), anti-Myc tag (Cat# 2272S, CST), anti-HA tag (Cat# 3724P, CST), anti-V5 (Cat# 13202S, CST), anti-GAPDH (Cat# 8884S, CST), anti-SAV1 (Cat# 3507S, CST), anti-GFP (Cat# A-11122, Thermo Fisher), anti-Lamin A/C (Cat# 2032, CST), anti-YAP (Cat#14074, CST, anti-YAP/TAZ (Cat#8418, CST), anti p-YAP (Cat# 4911, CST) anti Histone H3 (Cat# 4499, CST). See detailed information in Supplementary Table 3.

Antibodies employed were: anti-lamin A/C, goat polyclonal (Byorbit orb37882, Cambridge, UK) used at 1:100 dilution for IF and in situ proximity ligation assay (PLA); anti-lamin A/C (E1, Santa Cruz Biotechnology, Dallas, TX, USA) used at 1:500 dilution for IF and in situ proximity ligation assay (PLA) and 1:2000 for WB; anti-desmin (Abcam Ab15200 Cambridge, UK) used 1:1000 for IF and 1:2000 for PLA and WB; anti-desmin (Chemicon 1:400 for IF; phalloidin (Sigma-Aldrich, St. Louis, MO, USA) 1:1000 for IF; anti H3k9ac (Abcam, Cambridge, UK) 1:200 for IF; anti YAP (Santa Cruz Biotechnology, Dallas, TX, USA) 1:100 for IF; anti-emerin (Monosan, Uden, The Netherlands) 1:100 for IF; anti-FLAG tag (Sigma-Aldrich, St. Louis, MO, USA) 1:1000 for IF; anti-plectin 1 (D6A11, Cell signaling Technology, Danvers, MA, USA) 1:100 for IF and PLA.