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Chloroauric acid haucl4

Manufactured by Sinopharm
Sourced in China

Chloroauric acid (HAuCl4) is a chemical compound that consists of chlorine, hydrogen, and gold. It is a yellow, crystalline solid that is commonly used in various laboratory applications, particularly in the synthesis of gold nanoparticles and in the electroplating of gold.

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17 protocols using chloroauric acid haucl4

1

Synthesis and Characterization of Photocatalysts

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Phosphotungstic acid hydrate (PW12), Sodium tungstate dihydrate (Na2WO4·2H2O) were purchased from Macklin. The aluminum foil with 99.999% purity and 0.1 mm thickness came from General Research Institute for Nonferrous Metals (Beijing, China). Potassium hydroxide (KOH), hydrochloric acid (HCl), isopropanol, sodium chloride (NaCl), barium chloride (BaCl2), barium chloride (K2SO4), phosphoric acid (H3PO4), Tin (П) chloride (SnCl2), hydrogen peroxide (30% H2O2), ethanol, Chloroauric acid (HAuCl4) were from Sinopharm Chemical Reagent Co., Ltd. Potassium chloride (KCl), acetone, oxalic acid dehydrate and chromium trioxide (CrO3) were from Shanghai Ling Feng Chemical Reagent Co., Ltd. The resistivity of deionized water was 18.2 MΩ cm−1. The light source used in the experiments is 532 nm from Oxlasers and the light intensity is 50−500 mW cm−2.
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2

Fabrication of PLGA-Based Nanoparticles

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PLGA (lactide/glycolide =50:50, molecular weight =94,000 Da) was purchased from Daigang Biomaterial Co., Ltd. (Jinan, People’s Republic of China). Chloroauric acid (HAuCl4) was obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, People’s Republic of China). CTAB was obtained from Dibo Ltd. (Shanghai, People’s Republic of China). Ascorbic acid (AA), sodium borohydride (NaBH4), and silver nitrate (AgNO3) were purchased from Guobang Pharmaceutical Company (Shanxi, People’s Republic of China). GSH (purity >98%) was obtained from Genview Co., Ltd. (Beijing, People’s Republic of China). DTX was obtained from Meilun Biotechnological Company (Dalian, People’s Republic of China). Poly(vinyl alcohol) (PVA) was obtained from Kelong Chemical Reagent Company (Chengdu, People’s Republic of China). Both potassium permanganate (KMnO4) and 2-(N-morpholino) ethane-sulfonic acid (MES) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, People’s Republic of China). The Michigan Cancer Foundation – 7 (MCF-7) human breast cancer cell line was purchased commercially from the Cell Bank of the Chinese Academy of Sciences (Catalog No TCHu 74; Shanghai, People’s Republic of China). All the other chemicals were obtained from Sigma-Aldrich Co. Ltd (St Louis, MO, USA).
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3

Rapid Quantification of Phytochemicals

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PBZ, CAR, ovalbumin (OVA), bovine serum albumin (BSA), keyhole limpet hemocyanin (KLH), Tween-20, Freund’s adjuvants (complete and incomplete), culture media RPMI-1640, hypoxanthine aminopterin thymidine (HAT), hypoxanthine thymidine (HT), N-hydroxysuccinimide (NHS), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), 3,3,5,5-tetramethylbenzidine (TMB), polyethylene glycol 20,000 (PEG 20,000, 50%), goat anti-mouse IgG-horseradish peroxidase (goat anti-mouse IgG-HRP), N-propylethylenediamine (PSA), and graphitized carbon black (GCB) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium carbonate, N-N dimethylformamide (DMF), ethyl acetate, chloroauric acid (HAuCl4), and 3-hydroxytyramine hydrochloride (DA·HCl) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). The sample pad, absorbent paper, and polyvinylchloride (PVC) backing pads were purchased from Shanghai Kinbio Tech. Co., Ltd. (Shanghai, China). The nitrocellulose (NC) membrane was purchased from Sartorius Steadi Trading Co., Ltd. (Shanghai, China). Orange, grape, and cabbage mustard samples were purchased from the Tian Hong supermarket, (Nanchang, China). The 96-well polystyrene microplates and 6-well microplates were purchased from NEST Biotechnology Co., Ltd. (Wuhan, China).
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4

Analytical Characterization of Nanomaterials

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Metsulfuron-methyl, melamine, atrazine, and hexazinone were obtained from Sigma-Aldrich (St Louis, MO, USA). NaCl, MgCl2, glucose, L-cysteine, and vitamin C were purchased from Aladdin Industrial Corporation (Shanghai, China). Chloroauric acid (HAuCl4) was purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). All other reagents were analytical reagent grade.
A NanoDrop oneC spectrophotometer (Thermo Scientific, Waltham, MA, USA) was used to record UV-vis absorption spectra. A JEM-200CX transmission electron microscope (TEM, JEOL, Tokyo, Japan) was used for imaging particle aggregation. Vibrational spectra were obtained with a Fourier transform infrared spectrometer (FT-IR-8400, Shimadzu, Kyoto, Japan).
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5

AEP Inhibitor Evaluation for Neurodegeneration

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Chloroauric acid (HAuCl4) was purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Carboxylic acid PEG Thiol (SH-PEG-COOH) and SH-PEG-Cy5.5 were from Hunan Huateng Pharmaceutical Co., Ltd (Changsha City, China).The AK peptide (Ala–Ala–Asn–Cys–Lys) was customized by PHTD Peptide Co., Ltd (Zhengzhou, China). 2-Cyano-6-aminobenzothiazole (CABT) was customized by Shanghai Chemical Pharm-Intermediate Tech. Co., Ltd (Shanghai, China). Other chemical reagents were from Aladdin Bio-chem Technology Co., Ltd (Chengdu, China). The AEP inhibitor 7-Morpholin-4-yl-benzo[1,2,5]oxadiazol-4-ylamine (δ-secretase inhibitor 11, PubChem CID: 1095027) was purchased from J&K Scientific Ltd. Beijing, China. It was dissolved in dimethyl sulfoxide (DMSO) to make a stock solution and then diluted in a 0.9% NaCl solution containing gum Arabic for systemic treatment. The recombinant AEP and primary antibody for mature AEP were all from R&D Systems Inc (MN, USA). Amyloid β-protein fragment 25–35 (Aβ25–35) and Atorvastatin were from Sigma-Aldrich (St. Louis, MO, USA). The LysoTracker Green DND-26 for staining lysosomes in live cells was purchased from Thermo Fisher Scientific (Carlsbad, CA, US).
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6

Hyaluronic Acid-Based Theranostic Nanoparticles

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Sodium hyaluronic acid (Mw = 50 kDa) was obtained from Freda Biochem Co. Ltd (Shangdong China). Methacrylic anhydride and acrylyl chloride were obtained from Adamas Reagent Co. Ltd. Cystamine dihydrochloride and glutathione (GSH) were bought from Aladdin Industrial Corporation. Chloroauric acid (HAuCl4) and sodium borohydride (NaBH4) were supplied by Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). Doxorubic hydrochloride (DOX) was purchased from Shenzhen Main Luck Pharmaceuticals Inc. (Shenzhen, China). Potassium persulfate (K2S2O8) was recrystallized from deionized water. Dimethyl sulfoxide (DMSO) and triethylamine were distilled under reduced pressure from calcium hydride. All other reagents were of analytical grade and used without further purification. Murine hepatic H22 cell line, human pulmonary carcinoma cell line A549 and murine embryonic fibro-blast cell line NIH3T3 were provided by Shanghai Institute of Cell Biology (Shanghai, China). Male ICR mice (6–8 weeks old) were supplied by the Animal Center Laboratory of Nanjing Medical University (Nanjing, China). All animal experiments were performed in accordance with the Guidelines for Care and Use of Laboratory Animals of Nanjing University and approved by the Animal Ethics Committee of Drum-Tower Hospital.
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7

Enzyme-Catalyzed Sensor for Glutathione Detection

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Choline chloride (ChCl), acrylic acid (AA), ethylene glycol dimethacrylate (EDMA), benzoyl peroxid, N-hydroxysuccinimide (NHS), poly acetylimide (PEI), hydrazine hydrate, glutathione (GSH), N,N-dimethylaniline (DMA), and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) were supplied by Aladdin Reagent Co., Ltd. (Shanghai, China). The β-Lg was obtained from Sigma Aldrich Co., Ltd. (St. Louis, MO, USA). Graphene oxide was purchased from Suzhou Tanfeng Graphene Technology Co., Ltd. (Suzhou, China). Chloroauric acid (HAuCl4) was offered by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China) Screen-printed carbon electrode (SPCE) were commercially supplied by Yi Yue Co., Ltd., (Qingdao, China). All aqueous solutions were prepared with deionized water, and all other chemical used in this study were analytical grade.
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8

Lateral Flow Assay for Reproductive Hormones

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Acetone, N,N-dimethylformamide (DMF), sucrose, Tween-20, polyethylene glycol 20000 (PEG-20000), chloroauric acid (HAuCl4) and sodium citrate (Na3C6H5O7) were purchased from Sinopharm Chemical Reagent Co., Ltd (China). Nitrocellulose (NC) was purchased from Xingping Baotashan Coating Co., Ltd (Shaanxi, China). PVC backing plate, sample pad, conjugate pad, absorbent pad, α-hCG antibody, β-hCG antibody, β-LH antibody, α-LH antibody, goat anti-mouse IgG, commercial nitrocellulose (CNC) membrane and bovine serum albumin (BSA) were purchased from Shanghai JieYi Biotechnology Co., Ltd (Shanghai, China). Human chorionic gonadotropin (HCG), luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were purchased from Shanghai Sangon Biotech Co., Ltd (Shanghai, China).
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9

Synthesis of Graphene-Based Nanocomposites

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SiO2 was purchased from Degussa AG, Germany. GO was purchased from Solarbio, Beijing. Glucose was from Jinshan Chemical Test, Chengdu. CNTs dispersion in N-methyl-2-pyrrolidone (NMP) was obtained from Chengdu Organic Chemicals co., Ltd. Chinese academy of sciences, Chengdu. Chloroauric acid (HAuCl4) was purchased from Sinopharm Chemical Reagent co., Ltd, Shanghai. Thionine was from Shanghai Yuanye Bio-technology Co., Ltd, Shanghai. All other reagents and solvents were of analytical grade and commercially available and used without further purification. Ultra-pure water (18.25 MΩ) was used throughout.
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10

Synthesis and Characterization of Dendrimer-Gold Nanoparticles

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Amine-terminated generation 5 poly(amidoamine) dendrimer was purchased from Dendritech (Midland, MI, USA). Chloroauric acid (HAuCl4) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). FITC, acetic anhydride (Ac2O), and triethylamine (Et3N) was obtained from J&K Chemical Reagent Co., Ltd. (Beijing, China). Dimethyl sulfoxide (DMSO) was provided by Greagent. Sodium borohydride (NaBH4) was purchased from Kelong Reagent Co., Ltd. (Chongqing, China). All the chemicals were used without further purification. Dialysis bags with molecular weight cut-off (MWCO) of 14,000 Da were obtained from Shanghai Yuanye Biotechnology Corporation (Shanghai, China). U87MG cells (a human glioblastoma cell line) and L929 cells (a mouse fibroblast cell line) were obtained from the Institute of Biochemistry and Cell Biology, the Chinese Academy of Sciences (Shanghai, China). Minimum essential medium (MEM), trypsin containing EDTA, penicillin-streptomycin solution, and fetal bovine serum (FBS) were purchased from ThermoFisher Scientific (Waltham, MA, USA). Solutions of CCK-8, Hoechst 3334, Lyso-Tracker Red were obtained from Beyotime (Shanghai, China). De-ionized (DI) water (18.2 MΩ·cm) from a water purification system (Synergy, Millipore, MA, USA) was used in all the preparation processes.
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