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Mir 125b mimic

Manufactured by Qiagen
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The MiR-125b mimic is a laboratory reagent used for research purposes. It is designed to increase the expression of the miR-125b microRNA in biological samples. The core function of this product is to facilitate the study of miR-125b and its role in cellular processes.

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Lab products found in correlation

Dual luciferase reporter assay system, by Promega (1 mentions) Hiperfect transfection reagent, by Qiagen (1 mentions) Pluronic, by Merck Group (1 mentions)

2 protocols using mir 125b mimic

1

Luciferase Reporter Assay for miR-125b

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Human OA chondrocytes were co-transfected with luciferase reporter plasmids together with miR-125b mimic (Qiagen, Valencia, USA or Ambion, Austin, Texas, USA) using HiPerfect Transfection Reagent (Qiagen) as described previously13 (link). Dual-Luciferase Reporter Assay System (Promega, Madison, WI, USA) was used to analyze the luciferase activity after 24 hours post-transfection. Relative luciferase activity was measured by the ratio of reporter (firefly) to control (renilla) activity as instructed by the manufacturers (Promega).
Rasheed Z., Rasheed N., Abdulmonem W.A, & Khan M.I. (2019). MicroRNA-125b-5p regulates IL-1β induced inflammatory genes via targeting TRAF6-mediated MAPKs and NF-κB signaling in human osteoarthritic chondrocytes. Scientific Reports, 9, 6882.
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2

Pluronic Gel Delivery of miR-125b

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A 15% (w/v) solution of pluronic gel was made by heating sterile DNase/RNase-free PBS to 90°C and slowly mixing in the pluronic (Sigma) powder. Once all powder was dissolved, the magnetic stirring plate and gel were placed at 4°C until implantation. Immediately prior to surgery, miR-125b mimic (Qiagen) was mixed with the 15% pluronic gel at 4°C to obtain a final concentration of 10 μM.
Diaz Quiroz J.F., Tsai E., Coyle M., Sehm T, & Echeverri K. (2014). Precise control of miR-125b levels is required to create a regeneration-permissive environment after spinal cord injury: a cross-species comparison between salamander and rat. Disease Models & Mechanisms, 7(6), 601-611.
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