CP was supplied by Capot Chemical (Hangzhou, China), deionized water (18.2 MΩ.cm) was generated with a MilliQ System (Waters corp.). Potassium dihydrogen phosphate (H2KPO4), disodium phosphate (HNa2PO4) and methanol analytical grade were supplied by Panreac Quimica (Barcelona, Spain) and dimethyl sulfoxide (DMSO) was acquired from Fisher Scientific (Fisher Scientific, Leicestershire, UK). All other chemicals and reagents had suitable analytical grades (Fisher Scientific, Leicestershire, UK).
Milli q system
Manufactured by Waters Corporation
Sourced in United States
The Milli-Q system is a water purification system designed to produce high-quality ultrapure water. It utilizes a multi-stage filtration process to remove contaminants and impurities, providing water with consistent purity for various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Milli q ultrapure water, by Waters Corporation (2 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Luminol, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)
Human vegf165, by Thermo Fisher Scientific (1 mentions)
Tween 20, by Solarbio (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Chloroform, by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Phosphoric acid, by Thermo Fisher Scientific (1 mentions)
N hexane, by Thermo Fisher Scientific (1 mentions)
Spiramycin, by Merck Group (1 mentions)
1 2 dipalmitoyl sn glycero 3 phosphocholine, by Merck Group (1 mentions)
1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine popc, by Avanti Polar Lipids (1 mentions)
Nicolet is5 ftir spectrometer, by Thermo Fisher Scientific (1 mentions)
Du 730 uv vis spectrophotometer, by Beckman Coulter (1 mentions)
Xevo g2 q tof mass spectrometer, by Waters Corporation (1 mentions)
Sephadex lh 20, by GE Healthcare (1 mentions)
C 18 rp silica gel, by Nacalai Tesque (1 mentions)
12 protocols using milli q system
1
Phosphate Buffer Preparation Protocol
2
Magnetic Bead-Based Sandwich ELISA for CEA and VEGF
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Human VEGF165 was purchased from Peprotech Corporation (USA). CEA was obtained from Abcam Corporation (USA). Human CEA ELISA kit was acquired from Huaketai Biotechnology Co. Ltd. (Beijing, China). Human VEGF165 ELISA kit was from Miblo Co. Ltd. (Shanghai, China). Alkaline phosphatase-labeled streptavidin (ALP-SA) was purchased from Beyotime Biotechnology Co. Ltd. (Shanghai, China). Tween-20, Biotin, Dimethyl sulfoxide (DMSO) and Tris (hydroxymethyl) aminomethane-hydrochloric acid (Tris-HCl) were obtained from Solarbio Co. Ltd. (Beijing, China). HCl, NaCl, KCl, and NaOH were acquired from Kemiou Chemical Reagent Co. Ltd. (Tianjin, China). Streptavidin modified magnetic beads (MBs, 300 nm) were from Biomag Biotechnology Co. Ltd. (Wuxi, China). Luminol was acquired from Sigma corporation (USA). 3-(2-Spiroadamatane)-4-methoxy-4-(3-phosphoryloxy)phenyl-1,2-dioxetane (AMPPD) was from Huaxia reagent Co. Ltd. (Chengdu, China). 4-Phenylphenol (BIP) was purchased from Aladdin Co. Ltd. (Shanghai, China). Ultrapure water (18.3 MU cm) was obtained from a Milli-Q system (Waters, Milford, MA, USA) and used for all experiments.
All oligonucleotides employed in the work were synthesized and purified by Sangon Biotechnology Corporation (Shanghai, China) and the sequences are listed inTable 1 .
All oligonucleotides employed in the work were synthesized and purified by Sangon Biotechnology Corporation (Shanghai, China) and the sequences are listed in
3
Synthesis of Rhenium(I) Complexes with Pyridyl-Phosphine Ligands
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Compounds RePNBr (fac-P,N-{(C6H5)2(C5H4N)P}Re(CO)3Br), RePNNBr (fac-P,N-{(C6H5)2(C5H4N)(NH)P}Re(CO)3Br), and RePNTfO (fac-P,N-{(C6H5)2(C5H4N)P}Re(CO)3(TfO) where TfO = O3SCF3), were synthesized as described in the literature.38,40,41 (link) All solvents (Merck) were of spectroscopic or HPLC grade. Water was purified and deionized using a Waters Milli-Q system.
4
Spiramycin Quantification and M. synoviae Cultivation
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The current research was executed using spiramycin injectable solution (Mayco Spira®, 540,000 IU/mL corresponding to 168.75 mg/mL spiramycin as spiramycin adipate, Laboratorios Maymó, S.A., Barcelona, Spain); it was diluted with sterile 0.9% NaCl to make 56 mg/mL spiramycin for the animal experiment. The reference standard of spiramycin was procured from Sigma-Aldrich Co. (St. Louis, MO, USA). Phosphoric acid, methanol, n-hexane, acetonitrile, and potassium monobasic phosphate were manufactured by Thermo Fisher Scientific (Waltham, MA, USA). Ammonium acetate and chloroform were bought from Merck (Darmstadt, Germany). All utilized chemicals were of high-performance liquid chromatography (HPLC) analytical grade. Purification of water for HPLC was performed employing a Milli-Q system (Waters Corp., Milford, MA, USA). M. synoviae reference strain (MS WVU-1853) was provided by Animal Health Research Institute, Giza, Egypt. M. synoviae medium base was produced following the method of Frey et al. [58 ].
5
Flavonoid and Lipid Characterization
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Flavonoids (quercetin dihydrate, morin dihydrate and kaempferol hydrate) and lipids (1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) from Sigma and 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) from Avanti Polar Lipids (Alabaster, AL)) were used as received. Laurdan (6-dodecanoyl-2-(dimethylamine)-naphthalene) from Molecular Probes Invitrogen (Carlsbad, CA) and DPH (diphenylhexatriene) from Sigma were employed without further purification.
All solvents and reagents used were reagent grade, spectroscopic or HPLC quality. Water was purified and deionized using a Waters Milli-Q system.
All solvents and reagents used were reagent grade, spectroscopic or HPLC quality. Water was purified and deionized using a Waters Milli-Q system.
6
Detailed Analytical Techniques for Chemical Characterization
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Nuclear magnetic resonance (NMR) spectra were obtained using a Varian 400 spectrometer (Varian, Palo Alto, CA, USA) 400 MHz spectrometer operated at 400 MHz for 1H-NMR and at 100 MHz for 13C-NMR. High-resolution mass spectra data were measured on a Xevo G2 Q-TOF mass spectrometer (Waters, Milford, MA, USA). Fourier Transform Infrared (FT-IR) recorded on a Nicolet™ iS™ 5 FT-IR spectrometer (ThermoFisher Scientific, Madison, WI, USA) were used. Ultraviolet visible spectroscopy was performed using a DU 730 UV/Vis spectrophotometer (Beckman Coulter GmbH, North Rhine-Westphalia, Germany). Optical rotation and CD data are also given in the Supplementary file . Semi-preparative high performance liquid chromatography (HPLC) was performed on a system equipped with a Gilson 321 pump and Gilson 172 Diode Array Detector (Gilson, Madison, WI, USA) using YMC-pack Ph (250 × 20 mm) and YMC-pack Ph (250 × 10 mm) HPLC columns (YMC, Kyoto, Japan). Water was purified using a Milli-Q system (Waters Corporation, Milford, MA, USA). Column chromatography on C-18 RP silica gel (Cosmosil, Kyoto, Japan) and Sephadex LH-20 (GE Healthcare, Uppsala, Sweden) was conducted, and TLC analysis on silica gel 60 F254 plates (Merck, Darmstadt, Germany) was done. The spots were visualized by spraying with 10% aqueous H2SO4.
7
Contrast Agents in MRI Imaging
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Contrast agents were used as supplied by the manufacturer: linear GBCAs such as gadodiamide (Omniscan; GE Healthcare Buchler, Braunschweig, Germany), gadopentetate dimeglumine (Gd-DTPA, Magnevist; Bayer-Vital, Leverkusen, Germany), and gadobenate dimeglumine (Multihance; Bracco, Konstanz, Germany) as well as macrocyclic GBCAs such as gadobutrol (Gadovist; Bayer-Vital) and gadoterate meglumine (Gd-DOTA, Dotarem; Guerbet, Sulzbach/Taunus, Germany). All reagents used were of the highest purity. Deionized water was obtained from a Milli-Q system (Waters, Eschborn, Germany).
8
Chemiluminescent Assay Development
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Horseradish Peroxidase (HRP), luminol, HIOP, 4-IOP, 4-BOP, 4-IMP, 4-methoxyphenol (4-MYP), 2-iodophenol (2-IOP), 1-bromo-4-iodobenzene (4-BIB), 3-iodophenol (3-IOP), 4-phenylphenol (4-PYP), 4–4'-diiodobiphenyl (DIOP), and Trometamol (Tris) were purchased from Sigma-Aldrich (St. Louis MO, USA). Methanol, acetonitrile, acetone, DMF, N-hydroxysuccinimide (NHS), N, N'-dicyclohexyl carbodiimide (DCC), Tween 20 and hydrogen peroxide (H2O2) were supplied by Beijing Chemical Works (Beijing, China). All aqueous solutions and buffers were prepared using deionized water (resistivity > 18MΩ. cm), and all other chemicals and organic solvents were of analytical grade or better.
The white opaque 96-flat-bottomed well plates were purchased from Corning (COSTAR, NY, USA). Plates were washed with a DEM plate washer (Beijing Tuopu Analytical Instruments Co. Ltd., China). The pH of all buffer solutions was measured using a pH meter ((METTLER TOLEDO, China)). All chemiluminescent intensity measurements were performed with Multi+ Detection System with Instinct Software (Promega, WI, USA). Deionized water was purified with a MilliQ system (Waters, MA, USA).
The white opaque 96-flat-bottomed well plates were purchased from Corning (COSTAR, NY, USA). Plates were washed with a DEM plate washer (Beijing Tuopu Analytical Instruments Co. Ltd., China). The pH of all buffer solutions was measured using a pH meter ((METTLER TOLEDO, China)). All chemiluminescent intensity measurements were performed with Multi+ Detection System with Instinct Software (Promega, WI, USA). Deionized water was purified with a MilliQ system (Waters, MA, USA).
9
Cisplatin-Loaded Ophthalmic Film Formulation
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Cisplatin with purity higher than 99.9% was obtained from Sigma Aldrich (Saint Louis, MO, USA). Sodium hyaluronate (HA ophthalmic, batch A19270, molecular weight 1.33·106 Da) was kindly donated by Fidia Farmaceutici S.p.A. (Abano Terme, Italy). Polyvinyl alcohol was obtained from Nippon-Goshei (PVA; 83,000 Da; Osaka, Japan). Polyethylene glycol 1000 mono-stearate (PEG 1000S; batch 0001459543) was a kind gift of CRODA (Snaith, UK). Polyethylene glycol 200 (PEG 200) and sorbitol were purchased from ACEF (Fiorenzuola d’Arda, Italy). Water for film preparation was Milli-Q ultrapure water (18 MΩ cm, Milli-Q system, Waters Corp., Milford, MA, USA). All other chemicals, reagents and solvents were of analytical grade.
10
Aflatoxin Extraction and Quantification
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OTA, sodium tetraborate decahydrate (B4Na2O7 × 10 H2O), TWEEN® 20, sodium azide (NaN3) and ovalbumin (OVA) were obtained from Sigma-Aldrich (Milan, Italy). MAb clone 5E2 (Order No. 201052) was provided by Softflow Biotechnology (Pécs, Hungary). Glass culture tubes (10 × 75 mm) were supplied by VWR International S.R.L. (Milan, Italy). Glass microfiber filters (Whatman GF/A) and paper filters (Whatman N. 4) were purchased from Whatman (Maidstone, UK). Solid phase extraction columns Bond Elut NH2 (500 mg, 3 mL) were purchased by Agilent Technologies (Santa Clara, CA, USA). OchraTest™ immunoaffinity columns were provided by VICAM, a Water Business (Milford, MA, USA). All other chemicals and solvents were reagent grade or HPLC grade and were obtained from Sigma-Aldrich (Milan, Italy). Ultrapure water was produced by a Waters Milli-Q system (Waters, Milford, MA, USA).
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