Anti rb 4h1

Manufactured by Cell Signaling Technology

Anti‐Rb (4H1) is a mouse monoclonal antibody that recognizes the retinoblastoma protein (Rb). It is a key regulator of the cell cycle and plays a crucial role in the control of cell proliferation.

Automatically generated - may contain errors

Lab products found in correlation

Anti girdin, by R&D Systems (1 mentions) Anti histone h3 1b1b2, by Cell Signaling Technology (1 mentions) Anti histone h3 phospho s10, by Abcam (1 mentions) Anti cleaved parp1, by Abcam (1 mentions) Anti cleaved parp 1, by Cell Signaling Technology (1 mentions) Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) Anti p53, by Cell Signaling Technology (1 mentions) Anti γ tubulin, by Merck Group (1 mentions) Anti α tubulin, by Merck Group (1 mentions) Anti cdk2, by Santa Cruz Biotechnology (1 mentions) Anti ar n 20, by Santa Cruz Biotechnology (1 mentions) Chemiluminescent detection reagents ecl, by Cytiva (1 mentions) Secondary horseradish peroxidase conjugated antibodies, by Cytiva (1 mentions) Anti tcf4, by Merck Group (1 mentions) Anti egf receptor, by Cell Signaling Technology (1 mentions) Anti active β catenin, by Merck Group (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Anti p21, by Cell Signaling Technology (1 mentions) Anti p27, by BD (1 mentions)

Close spelling variants of this product

Anti-Rb (46 citations) Rb (4H1, (4 citations)

2 protocols using anti rb 4h1

Comprehensive Protein Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following Abs were used in this study: anti‐Girdin (R&D Systems), anti‐Girdin (IBL), anti‐Girdin phospho S1647 (ECM Biosciences), anti‐histone H3 (1B1B2) (Cell Signaling Technology), anti‐histone H3 phospho S10 (Abcam), anti‐histone H3 phospho S28 (Abcam), anti‐cleaved PARP1 (Abcam), anti‐cleaved PARP1 (Cell Signaling Technology), anti‐Rb phospho Ser795 (New England BioLabs), anti‐Rb (4H1) (Cell Signaling Technology), anti‐p53 (Cell Signaling Technology), anti‐p53 phospho S15 (Cell Signaling Technology), anti‐p53 phospho S46 (Cell Signaling Technology), anti‐Mad2 (C‐10) (Santa Cruz Biotechnology), anti‐α‐tubulin (Sigma‐Aldrich), anti‐γ‐tubulin (Sigma‐Aldrich), Alexa Fluor 488 goat anti‐mouse IgG (Thermo Fisher Scientific), Alexa Fluor 488 goat anti‐rabbit IgG (Thermo Fisher Scientific), rabbit anti‐sheep IgG (H + L), Human SP ads‐HRP (Southern Biotech), and rabbit anti‐rat IgG H&L (HRP) (Abcam) Abs.

Chen C., Enomoto A., Weng L., Taki T., Shiraki Y., Mii S., Ichihara R., Kanda M., Koike M., Kodera Y, & Takahashi M. (2020). Complex roles of the actin‐binding protein Girdin/GIV in DNA damage‐induced apoptosis of cancer cells. Cancer Science, 111(11), 4303-4317.

+ Open protocol

+ Expand

Western Blot Profiling of Cellular Pathways

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blotting was performed as previously described [7 (link)]. Whole-cell lysates collected from 100,000 cells were used per lane. Antibodies used were: anti-AR (N-20, Santa Cruz; Santa Cruz, CA); anti-β-Actin (Cell Signaling; Beverly, MA); anti-ΔNp63 (4A4, Santa Cruz); anti-p21 (Cell Signaling); anti-p27 (BD Transduction Labs; San Diego, CA); anti-RB (4H1, Cell Signaling); anti-phospho-RB (Ser 608, Cell Signaling); anti-SKP2 (Zymed; San Francisco, CA); anti EGF receptor (#2232, Cell Signaling); anti-IGF-type 1 receptor (#3018; Cell Signaling); anti-CDK-2 (H-298; Santa Cruz); anti-Cyclin D1 (Upstate Biotechnology; Lake Placid, NY); anti-c-MYC (Calbiochem; San Diego, CA); anti-TCF-4 (05-511, Millipore; Billerica, MA); anti-active β-Catenin (05-665, Millipore); anti-phospho-S552 β-catenin (#9566; Cell Signaling); anti-FOXP3 (mAbcam 450, Abcam; Cambridge, MA). All secondary horseradish peroxidase-conjugated antibodies and chemiluminescent detection reagents (ECL) were purchased from Amersham Biosciences (Piscataway, NJ).

Antony L., van der Schoor F., Dalrymple S.L, & Isaacs J.T. (2014). Androgen Receptor (AR) Suppresses Normal Human Prostate Epithelial Cell Proliferation via AR/β-catenin/TCF-4 Complex Inhibition of c-MYC Transcription. The Prostate, 74(11), 1118-1131.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!