Log In Sign up
The largest database of trusted experimental protocols

Mouse il 6 elisa kit

Manufactured by RayBiotech
Visit Supplier
Sourced in United States

The Mouse IL-6 ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse interleukin-6 (IL-6) levels in cell culture supernatants, serum, and plasma samples.

Automatically generated - may contain errors

Lab products found in correlation

Mouse tnf alpha elisa kit, by RayBiotech (2 mentions) Human il 6 elisa kit, by RayBiotech (2 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions) Vitamin d3, by Solarbio (1 mentions) Total cholestenone content assay kit, by Solarbio (1 mentions) Total sod activity detection kit, by Beyotime (1 mentions) Anti fas antibody, by Abcam (1 mentions) Anti zo1 tight junction protein antibody, by Abcam (1 mentions) Anti occludin antibody, by Abcam (1 mentions) Triglyceride content assay kit, by Solarbio (1 mentions) Oil red o, by Merck Group (1 mentions) Superreal premix plus sybr green, by Tiangen Biotech (1 mentions) Bca protein concentration assay kit, by Beyotime (1 mentions) Cdna synthesis kit, by Tiangen Biotech (1 mentions) Quantikine elisa kit, by R&D Systems (1 mentions) Msd technology, by Mesoscale (1 mentions) Enzychrom creatine kinase assay kit, by BioAssay Systems (1 mentions) Human rantes ccl5 elisa kit, by RayBiotech (1 mentions) Human cytokine array c5, by RayBiotech (1 mentions) Triglyceride colorimetric assay kit, by Cayman Chemical (1 mentions)

Close spelling variants of this product

Mouse ELISA kit ELISAs

13 protocols using mouse il 6 elisa kit

1

Investigating Liver Inflammation and Metabolism

Check if the same lab product or an alternative is used in the 5 most similar protocols
Vitamin C (Tianjin Kingyork, H12020392), Vitamin D3 (Beijing Solarbio Science & Technology, V8070), Mouse TNF-alpha ELISA Kit (RayBiotech, P06804), Mouse IL-6 ELISA Kit (RayBiotech, P08505), Mouse IL-1β ELISA Kit (Shanghai Tongwei Biotechnology, TW56961), Mouse insulin ELISA Kit (Shanghai Tongwei Biotechnology, TW085566), Mouse endotoxin (ET) ELISA Kit (Jiangsu Meimian, MM-0369M1), Total Cholestenone Content Assay Kit (Beijing Solarbio Science & Technology, BC 1985), Triglyceride Content Assay Kit (Beijing Solarbio Science & Technology, BC0625), Total SOD activity detection kit (Shanghai Beyotime Biotechnology, S0101S), Lipid oxidation detection kit (Shanghai Beyotime Biotechnology, S0131S), BCA protein concentration assay kit (Shanghai Beyotime Biotechnology, P0010), Oil red O (Sigma, O0625), Anti-FXR1 antibody (Abcam, ab155124), BSEP Monoclonal antibody (Proteintech Group, 67512-1-Ig), Anti-Fas antibody (Abcam, ab271016), Anti-ZO1 tight junction protein antibody (Abcam, ab221547), Anti-Occludin antibody (Abcam, ab222691), Anti-SLC10A2/ASBT antibody (Abcam, ab203205), TRIzol Reagent (Thermo Fisher, 15596026), cDNA synthesis kit (Beijing Tiangen Biotech, KR118), and SuperReal PreMix Plus (SYBR Green) (Beijing Tiangen Biotech, FP205) were used.
Chen Q., Zhao L., Mei L., Zhao X., Han P., Liu J., Meng C., Li R., Zhong R., Wang K, & Li J. (2023). Vitamin C and vitamin D3 alleviate metabolic-associated fatty liver disease by regulating the gut microbiota and bile acid metabolism via the gut-liver axis. Frontiers in Pharmacology, 14, 1163694.
+ Open protocol
+ Expand
2

Quantification of Liver Cytokines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tnf protein levels from liver homogenates were measured using a Quantikine-Elisa-Kit from R&D Systems (Oxon, UK). IL6 protein levels were measured using a mouse IL6 Elisa kit from RayBiotech (GA, US). The procedures closely followed the manufacturer’s instructions. The detection limit was 16 pg/ml for Tnf and 2 pg/ml for IL6 respectively. The homogenization buffer was tested as a negative control.
Yuan D., Huang S., Berger E., Liu L., Gross N., Heinzmann F., Ringelhan M., Connor T.O., Stadler M., Meister M., Weber J., Öllinger R., Simonavicius N., Reisinger F., Hartmann D., Meyer R., Reich M., Seehawer M., Leone V., Höchst B., Wohlleber D., Jörs S., Prinz M., Spalding D., Protzer U., Luedde T., Terracciano L., Matter M., Longerich T., Knolle P., Ried T., Keitel V., Geisler F., Unger K., Cinnamon E., Pikarsky E., Hüser N., Davis R.J., Tschaharganeh D.F., Rad R., Weber A., Zender L., Haller D, & Heikenwalder M. (2017). Kupffer Cell-Derived Tnf Triggers Cholangiocellular Tumorigenesis through JNK due to Chronic Mitochondrial Dysfunction and ROS. Cancer cell, 31(6), 771-789.e6.
+ Open protocol
+ Expand
3

Ketogenic Diet Alters Inflammatory Cytokines

Check if the same lab product or an alternative is used in the 5 most similar protocols
To study KD effects on inflammatory cytokines, 12 weeks old male mice were randomly assigned to either chow, KD or HFD for 3 days and 16 weeks. Mice were fasted for 6 h and injected glucose 30 min, or without glucose injections before tissue and blood sampling. Circulating IL-6 concentration, IL-6 in liver, mesenteric fat, and ingWAT lysate were measured by using Mouse IL-6 ELISA Kit (RayBiotech, #ELM-IL6, Luzern, Switzerland). Liver cholesterol was measured by Cobas Roche (Hitachi Kit #11877771, Roche Diagnostics International). Plasma cytokine Interferon gamma (IFN-γ), interleukin 1 beta (IL-1β), interleukin 2 (IL-2), interleukin 5 (IL-5), interleukin 6 (IL-6), interleukin 10 (IL-10), interleukin-12 (IL-12p70), tumor necrosis factor alpha (TNF-α), and keratinocyte chemoattractant (KC)/growth-regulated oncogene (GRO) chemokines and pro-inflammatory chemokines were measured using the MSD technology (Meso Scale Discovery, Gaithersburg, MD, USA). All analyses were carried out according to manufacturer's protocols.
Long F., Bhatti M.R., Kellenberger A., Sun W., Modica S., Höring M., Liebisch G., Krieger J.P., Wolfrum C, & Challa T.D. (2023). A low-carbohydrate diet induces hepatic insulin resistance and metabolic associated fatty liver disease in mice. Molecular Metabolism, 69, 101675.
+ Open protocol
+ Expand
4

Quantification of Serum IL-6 and CK

Check if the same lab product or an alternative is used in the 5 most similar protocols
The IL-6 and CK levels in serum were determined using a mouse IL-6 ELISA Kit (Ray Biotech, GA, USA) and EnzyChromTM Creatine Kinase Assay Kit (BioAssay Systems, CA, USA), respectively, according to manufacturer's instructions. The following protocol was followed for measuring the serum CK levels: R1 reagent buffer (100 μl) containing substrate was added to 6 μl of serum sample, and then incubated for 10 min at 37ºC. Following incubation, absorbance was determined at 340 nm wavelength. Serum IL-6 levels were measured as follows: Sample (100 μl) was added into 96-well plate coated with anti-IL-6 antibody and was incubated for 2.5 h at R/T. Next, the sample was incubated with biotin antibody at R/T for 1 h, followed by incubation with streptavidin incubation for 45 min. Absorbance was measured at 450 nm after 30 min of incubation with TMB One-Step Substrate.
Lee Y.I, & Leem Y.H. (2019). Acid sphingomyelinase inhibition alleviates muscle damage in gastrocnemius after acute strenuous exercise. Journal of Exercise Nutrition & Biochemistry, 23(2), 1-6.
+ Open protocol
+ Expand
5

Profiling Cytokine Secretion in Breast Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Quantibody Mouse Cytokine Array (QAM‐CYT‐Q‐2000) was purchased from RayBiotech and used to analyze conditioned medium harvested from 4T1.V and 4T1.ΔC lines as per the supplier's instructions. To generate conditioned media, 3 × 106 cells were plated in 10‐cm tissue culture dishes and incubated in serum‐free OptiMem for 48 hours prior to collection. Conditioned medium was filtered to remove cell debris and particulates prior to analysis. To validate protein expression profiles for IL‐6, conditioned media from 4T1.V and 4T1.ΔC was harvested and analyzed using a mouse IL‐6 ELISA kit (RayBiotech) as per the manufacturer's instructions. Conditioned media from MDA.V and MDA.A2KD cells were analyzed using a human IL‐6 ELISA kit (RayBiotech).
Vaught D.B., Merkel A.R., Lynch C.C., Edwards J., Tantawy M.N., Hilliard T., Wang S., Peterson T., Johnson R.W., Sterling J.A, & Brantley‐Sieders D. (2021). EphA2 Is a Clinically Relevant Target for Breast Cancer Bone Metastatic Disease. JBMR Plus, 5(4), e10465.
+ Open protocol
+ Expand
6

Quantification of Soluble IL-6R and IL-6 in Kidney

Check if the same lab product or an alternative is used in the 5 most similar protocols
Soluble IL-6R was quantified in plasma and tissue lysates from sham and UUO mouse kidneys using a mouse IL-6R ELISA kit (Raybiotech, Norcross, GA, USA). Plasma IL-6 was quantified using a mouse IL-6 ELISA kit (Raybiotech, Norcross, GA, USA). Renal lysate and plasma samples were diluted in reagent diluent and subjected to ELISA analysis according to the manufacturer's protocol.
Chen W., Yuan H., Cao W., Wang T., Chen W., Yu H., Fu Y., Jiang B., Zhou H., Guo H, & Zhao X. (2019). Blocking interleukin-6 trans-signaling protects against renal fibrosis by suppressing STAT3 activation. Theranostics, 9(14), 3980-3991.
+ Open protocol
+ Expand
7

Tail Suspension and Vitamin D3 in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were divided into five groups: control, tail suspension, tail suspension+1,25(OH)2D3, tail suspension+HMB, and tail suspension+1,25(OH)2D3+HMB (each n = 4). After one week of pretreatment, the mice were subjected to two weeks of tail suspension. The serum IL-6 level was determined using a mouse IL-6 ELISA Kit (Ray Biotech, GA, USA) according to the manufacturer’s protocol.
Yakabe M., Ogawa S., Ota H., Iijima K., Eto M., Ouchi Y, & Akishita M. (2018). Inhibition of interleukin-6 decreases atrogene expression and ameliorates tail suspension-induced skeletal muscle atrophy. PLoS ONE, 13(1), e0191318.
+ Open protocol
+ Expand
8

Cytokine Quantification Using ELISA Kits

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cytokine quantification was measured following the manufacturer’s instructions, IFN-γ (RayBio® Mouse IFN-gamma ELISA Kit, Catalog #: ELM-IFNg); TNF-α (RayBio® Mouse TNF-alpha ELISA Kit, Catalog #: ELM-TNFa); IL-6 (RayBio® Mouse IL-6 ELISA Kit Catalog #: ELM-IL-6); IL-10 (ELISA assay using Ray Bio® ELISA Kits, Catalog #: ELM-IL-10). The concentration of the cytokines was determined by measuring color intensity at 450 nm using a microplate reader.
Sadek A.S., Farghaly D.S., Kadada H, & Mashaal A. (2024). Immunomodulatory potential of Sarcophaga argyostoma larval hemolymph as a natural alternative to berenil in treating Trypanosoma evansi in vivo. Scientific Reports, 14, 6972.
+ Open protocol
+ Expand
9

Investigating Notch1 Signaling in Cell Secretome

Check if the same lab product or an alternative is used in the 5 most similar protocols
2 ×106 cells expressing either shGFP or shNotch1 were seeded in 10 cm plates with 10% FBS DMEM complete media, and cultured for one day. Cells were then washed three times with PBS before addition of serum free media. After three days in culture, supernatants were collected and the volumes were adjusted by total protein amount in cell lysates. Chemokines were quantified as pg/μg total protein in whole cell lysates. C-arrays and ELISA assays were performed according to manufacturer’s instructions. Human Cytokine Array C5, Mouse Cytokine Array C3, Human IL-8 ELISA Kit (ELH-IL8-1), Human RANTES (CCL5) ELISA Kit (ELH-RANTES-1), Mouse RANTES ELISA Kit (ELM-RANTES-1), Human IL-6 ELISA Kit (ELH-IL6-1), Mouse IL-6 ELISA Kit (ELM-IL6-1), Mouse MIP-2 ELISA Kit (ELM-MIP2-1) were all from Raybiotech.
Qiu H., Zmina P.M., Huang A.Y., Askew D, & Bedogni B. (2018). Inhibiting Notch1 enhances immunotherapy efficacy in melanoma by preventing Notch1 dependent immune suppressive properties. Cancer letters, 434, 144-151.
+ Open protocol
+ Expand
10

Plasma Biomarker Measurement Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma corticosterone concentrations were measured using the Mouse Corticosterone ELISA Kit (Arbor Assays®, Ann Arbor, Michigan) according to the manufacturer’s instructions. Plasma and liver homogenate triglyceride concentrations were measured using the Triglyceride Colorimetric Assay Kit (Cayman Chemical, Ann Arbor, Michigan) according to the manufacturer’s instructions. Frozen livers were homogenized in NP40 Substitute Assay Reagent from the Triglyceride Colorimetric Assay Kit, centrifuged at 10,000 × g for 10 min, and triglycerides were measured in the supernatant according to the manufacturer’s instructions. Plasma interleukin 6 (IL6) concentrations were determined using a Mouse IL-6 ELISA Kit (RayBio®, Norcross, Georgia) following the manufacturer’s instructions.
Henderson C.G., Turner D.L, & Swoap S.J. (2021). Health Effects of Alternate Day Fasting Versus Pair-Fed Caloric Restriction in Diet-Induced Obese C57Bl/6J Male Mice. Frontiers in Physiology, 12, 641532.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!